Artículos y presentaciones en Congresos
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Examinando Artículos y presentaciones en Congresos por Autor "Antonelli, Cristian Javier"
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Acceso Abierto Guapa bajo el agua: Lotus tenuis(2017) Bailleres, Matias; Campestre, María Paula; Antonelli, Cristian JavierEsta forrajera no sólo resiste condiciones de encharcamiento, sino que con técnicas de manejo, se potencia - Artículo
Acceso Abierto Lotus spp: Biotechnological strategies to improve the bioeconomy of lowlands in te Salado River Basin (Argentina)(2016) Antonelli, Cristian Javier; Calzadilla, Pablo I.; Escaray, Francisco José; Babuin,Maria F.; Campestre, María Paula; Rocco, Rubén; Bordenave, César D.; Perea García, Ana; Nieva, Amira S.; Llames, Maria E.; Maguire, Vanina; Melani, Gustavo; Serena, Daniel; Bailleres, Matías; Carrasco, Pedro; Paolocci, Francesco; Garriz, Andrés; Menéndez, Ana; Ruiz, Oscar AdolfoThe Salado River Basin region is the most important livestock breeding area in Argentina, wherethe Lotus species has been traditionally cultivated as forages. Nearly 60% of their land surface is dominated by salt-affected soils with severe constraints for crop cultivation. In order to cope with that limitation, farmers have utilized species such as non-native L. tenuis (ex- Lotus glaber), which shows a very good adaptation. As a result, inter-seeding of L. tenuis has been proposed as a strategy of choice for improving forage production in marginal areas. The increase in soil quality by these means is achieved by an increment of the organic matter content, improvement of soil fertility as well as microbial biodiversity. Thus, the introduction of L. tenuis and/or other Lotus genotypes could have enormous benefits for similar constrained lands around the world. We are developing an integrated analysis of the changes that occur in soils under legume production. We will not only analyze the microbial diversity associated, but also soil physical and chemical characteristics and the impact of different legume-microbes association on mitigation of GHG emissions. In addition, we are identifying the main genetic determinants associated with interesting agronomic traits such as plant tolerance against biotic and abiotic stresses and the content of condensed tannins. Our future and present research will build a solid base for the improvement of agronomicallyimportant species and the development of better strategies for the management of constrained lands such as the lowlands in the Argentinean Pampas. - Artículo
Acceso Abierto Response to long-term NaHCO3-derived alkalinity in model Lotus japonicus ecotypes Gifu B-129 and Miyakojima MG-20: transcriptomic profiling and physiological characterization(2014) Campestre, María Paula; Babuin, Maria Florencia; Rocco, Rubén; Bordenave, Cesar Daniel; Escaray, Francisco José; Antonelli, Cristian Javier; Calzadilla, Pablo; Gárriz, Andrés; Serna, Eva; Carrasco, Pedro; Ruiz, Oscar Adolfo; Menéndez, Ana B.The current knowledge regarding transcriptomic changes induced by alkalinity on plants is scarce and limited to studies where plants were subjected to the alkaline salt for periods not longer than 48 h, so there is no information available regarding the regulation of genes involved in the generation of a new homeostatic cellular condition after long-term alkaline stress. Lotus japonicus is a model legume broadly used to study many important physiological processes including biotic interactions and biotic and abiotic stresses. In the present study, we characterized phenotipically the response to alkaline stress of the most widely used L. japonicus ecotypes, Gifu B-129 and MG-20, and analyzed global transcriptome of plants subjected to 10 mM NaHCO3 during 21 days, by using the Affymetrix Lotus japonicus GeneChipH. Plant growth assessment, gas exchange parameters, chlorophyll a fluorescence transient (OJIP) analysis and metal accumulation supported the notion that MG-20 plants displayed a higher tolerance level to alkaline stress than Gifu B-129. Overall, 407 and 459 probe sets were regulated in MG-20 and Gifu B-129, respectively. The number of probe sets differentially expressed in roots was higher than that of shoots, regardless the ecotype. Gifu B-129 and MG-20 also differed in their regulation of genes that could play important roles in the generation of a new Fe/Zn homeostatic cellular condition, synthesis of plant compounds involved in stress response, protein-degradation, damage repair and root senescence, as well as in glycolysis, gluconeogenesis and TCA. In addition, there were differences between both ecotypes in the expression patterns of putative transcription factors that could determine distinct arrangements of flavonoid and isoflavonoid compounds. Our results provided a set of selected, differentially expressed genes deserving further investigation and suggested that the L. japonicus ecotypes could constitute a useful model to search for common and distinct tolerance mechanisms to long-term alkaline stress response in plants.