Artículos y presentaciones en Congresos
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Examinando Artículos y presentaciones en Congresos por Autor "Brutti, Cristina B."
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Acceso Abierto Caracterización parcial de una proteinasa coagulante de la leche aislada del alcaucil (Cynara scolymus L., Asteraceae)(1997) Llorente, Berta Elizabet; Brutti, Cristina B.; Caffini, Néstor Oscar; Natalucci, Claudia LuisaThe presence of proteinases in Cynara scolyrnus L. ( artichoke ) has been investigated by determining the proteolytic and milk clotting activities of crude extracts of different parts of the inflorescence in various stages of development, as well as of leaves and roots. Although all the preparations showed a certain extent of proteolytic activity, only those of adult leaves, pappus, and immature and mature flowers were able to clot milk. The extract of the upper (violet) part of mature flowers exhibited optimum activity at acid pH values (90% of maximum activity at pH 3.5 - 5.0) which was strongly inhibited by pepstatine A, suggesting the presence of aspartic proteinases. This extract had a low thermal stability at temperatures above 45ºC, which could be a useful property in cheese making process, as it could be quickly inactivated by moderate heating.