IIB-INTECH

URI permanente para esta comunidad

https://digital.cic.gba.gob.ar/handle/11746/146
El Instituto de Investigaciones Biotecnológicas - Instituto Tecnologico Chascomus es una institución dependiente de la Universidad Nacional de San Martín y del Consejo Nacional de Investigaciones Científicas y Técnicas generada a partir de la fusión del Instituto de Investigaciones Biotecnológicas (IIB), con sede en el Campus Miguelete de la UNSAM, y el Instituto Tecnológico de Chascomús (INTECH). Este instituto está dedicado a la investigación científica en bioquímica, biología molecular, biología celular, neurobiología, microbiología e inmunología, entre otras.

Director: Dr. Carlos Frasch

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Examinando IIB-INTECH por Autor "Antonelli, Cristian Javier"

Mostrando1 - 5 de 5
  • Artículo
    Acceso Abierto
    Guapa bajo el agua: Lotus tenuis
    (2017) Bailleres, Matias; Campestre, María Paula; Antonelli, Cristian Javier
    Esta forrajera no sólo resiste condiciones de encharcamiento, sino que con técnicas de manejo, se potencia
  • Informe de becario
    Acceso Abierto
    Informe científico de Beca de Estudio: Antonelli, Cristian Javier (2011-2012) 
    (2011) Antonelli, Cristian Javier
    Trabajos Realizados: Docencia: Evaluación fisiológica, morfológica y anatómica de Lotus spp. bajo estrés por inundación, su relación con microorganismos simbiontes mutualistas y su importancia como criterio de mejora. Divulgación:Colaboración en las actividades de divulgación para productores, junto con el Ing. Agr. Bailleres de la Estación Experimental Integrada de Manantiales (INTA-MAA). Otros:Colaboración en la redacción de una propuesta presentada ante el HCD de Chascomús (Proyecto de Resolución Nº 1521:Desgravación impositiva a productores en ambientes marginales) fundamentado en las técnicas de promoción de Lotus spp. en bajos salinos alcalinos.
  • Informe de becario
    Acceso Abierto
    Informe científico de Beca de Perfeccionamiento: Antonelli, Cristian Javier (2015)
    (2015) Antonelli, Cristian Javier
    A continuación se presentan los ensayos realizados durante el período transcurrido de las 2 becas otorgadas por la CIC (De estudio y de Perfeccionamiento): - Ensayo comparativo de rendimiento de cultivares de Lotus tenuis. - Promoción continua de Lotus tenuis con glifosato vs "manejo vivo" alternado. - Evaluación a campo de cepas de Mesorhizobium loti formuladas en inoculantes con soporte líquido y con soporte sólido (turba). - Bacterias promotoras de crecimiento frente a suelos salinos-alcalinos. - Ensayo de intensidad y frecuencia de corte de L. tenuis. - Germinación-emergencia de Lotus spp. en inundación -Comportamiento fisiológico, morfológico y anatómico de diferentes especies del género Lotus y del híbrido interespecífico, frente a condiciones de inundación. - Evaluación de las respuestas de la simbiosis mutualista con bacterias fijadoras de N2 frente al estrés por inundación en genotipos con diferente tolerancia a dicho estrés. - Respuesta de Lotus spp. frente a estrés por inundación parcial, a salinidad y a la combinación de ambos estreses. - Respuesta metabolómica de dos especies cultivadas del género Lotus frente a estrés por inundación parcial.
  • Artículo
    Acceso Abierto
    Lotus spp: Biotechnological strategies to improve the bioeconomy of lowlands in te Salado River Basin (Argentina)
    (2016) Antonelli, Cristian Javier; Calzadilla, Pablo I.; Escaray, Francisco José; Babuin,Maria F.; Campestre, María Paula; Rocco, Rubén; Bordenave, César D.; Perea García, Ana; Nieva, Amira S.; Llames, Maria E.; Maguire, Vanina; Melani, Gustavo; Serena, Daniel; Bailleres, Matías; Carrasco, Pedro; Paolocci, Francesco; Garriz, Andrés; Menéndez, Ana; Ruiz, Oscar Adolfo
    The Salado River Basin region is the most important livestock breeding area in Argentina, wherethe Lotus species has been traditionally cultivated as forages. Nearly 60% of their land surface is dominated by salt-affected soils with severe constraints for crop cultivation. In order to cope with that limitation, farmers have utilized species such as non-native L. tenuis (ex- Lotus glaber), which shows a very good adaptation. As a result, inter-seeding of L. tenuis has been proposed as a strategy of choice for improving forage production in marginal areas. The increase in soil quality by these means is achieved by an increment of the organic matter content, improvement of soil fertility as well as microbial biodiversity. Thus, the introduction of L. tenuis and/or other Lotus genotypes could have enormous benefits for similar constrained lands around the world. We are developing an integrated analysis of the changes that occur in soils under legume production. We will not only analyze the microbial diversity associated, but also soil physical and chemical characteristics and the impact of different legume-microbes association on mitigation of GHG emissions. In addition, we are identifying the main genetic determinants associated with interesting agronomic traits such as plant tolerance against biotic and abiotic stresses and the content of condensed tannins. Our future and present research will build a solid base for the improvement of agronomicallyimportant species and the development of better strategies for the management of constrained lands such as the lowlands in the Argentinean Pampas.
  • Artículo
    Acceso Abierto
    Response to long-term NaHCO3-derived alkalinity in model Lotus japonicus ecotypes Gifu B-129 and Miyakojima MG-20: transcriptomic profiling and physiological characterization
    (2014) Campestre, María Paula; Babuin, Maria Florencia; Rocco, Rubén; Bordenave, Cesar Daniel; Escaray, Francisco José; Antonelli, Cristian Javier; Calzadilla, Pablo; Gárriz, Andrés; Serna, Eva; Carrasco, Pedro; Ruiz, Oscar Adolfo; Menéndez, Ana B.
    The current knowledge regarding transcriptomic changes induced by alkalinity on plants is scarce and limited to studies where plants were subjected to the alkaline salt for periods not longer than 48 h, so there is no information available regarding the regulation of genes involved in the generation of a new homeostatic cellular condition after long-term alkaline stress. Lotus japonicus is a model legume broadly used to study many important physiological processes including biotic interactions and biotic and abiotic stresses. In the present study, we characterized phenotipically the response to alkaline stress of the most widely used L. japonicus ecotypes, Gifu B-129 and MG-20, and analyzed global transcriptome of plants subjected to 10 mM NaHCO3 during 21 days, by using the Affymetrix Lotus japonicus GeneChipH. Plant growth assessment, gas exchange parameters, chlorophyll a fluorescence transient (OJIP) analysis and metal accumulation supported the notion that MG-20 plants displayed a higher tolerance level to alkaline stress than Gifu B-129. Overall, 407 and 459 probe sets were regulated in MG-20 and Gifu B-129, respectively. The number of probe sets differentially expressed in roots was higher than that of shoots, regardless the ecotype. Gifu B-129 and MG-20 also differed in their regulation of genes that could play important roles in the generation of a new Fe/Zn homeostatic cellular condition, synthesis of plant compounds involved in stress response, protein-degradation, damage repair and root senescence, as well as in glycolysis, gluconeogenesis and TCA. In addition, there were differences between both ecotypes in the expression patterns of putative transcription factors that could determine distinct arrangements of flavonoid and isoflavonoid compounds. Our results provided a set of selected, differentially expressed genes deserving further investigation and suggested that the L. japonicus ecotypes could constitute a useful model to search for common and distinct tolerance mechanisms to long-term alkaline stress response in plants.