Examinando por Autor "Corva, Santiago"
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Acceso Abierto Hematologyand Serum Biochemistryof Free-Ranging Nutria (Myocastor Coypus)(American Association of Zoo Veterinarians, 2012) Martino, Pablo Eduardo; Aráuz, Sandra M.; Anselmino, Fernando; Cisterna, Cecilia; Silvestrini, María P.; Corva, Santiago; Hozbor, FedericoInformation on reference blood values in the literature is lacking for many wild rodents. In this study, comprehensive reference intervals (RIs) for a wide range of analytes from 101 healthy free-ranging nutria were determined. Animals were captured in Buenos Aires, Argentina (378509S, 578349W), and southward (388609S, 588239W), encompassing major biotopes of agricultural pampas with dunes and grassland steppes on the east coast. Traps were set at locations with high-density nutria populations (i.e., those areas that showed signs of movement, territorial marking, or feeding activities). Although the small sample size limits the interpretation of these findings, RIs were determined by a robust method using the central 95th percentile. In nutria, the RI range varied greatly for the leukocyte differentials, with mature neutrophils: 3,907–5,544/ll for females and 3,744–5,900/ll for males; band neutrophils: 0–10/ll for females and 3–18/ll for males; lymphocytes: 4,213– 5,940/ll for both sexes combined; monocytes: 165–402/ll for both sexes combined; eosinophils: 13–91/ll for females and 108–165/ll for males; and basophils: 0–87/ll for both sexes combined. Platelet concentration was 543–727 3 109/L for both sexes combined. There was also a wide RI range for biochemistry values for some enzymes, such as alkaline phosphatase: 200–399 IU/L for both sexes combined; cholinesterase: 762–1,407 IU/L for females and 763–1,284 IU/L for males; creatine kinase: 182–552 IU/L for females and 162–451 IU/L for males; amylase: 853–1,865 IU/L for females and 779–1,293 IU/L for males; and glucose concentration 120.2– 180.6 mg/dl for both sexes combined. Conversely, there was not a wide pooled RI range for calcium: 7.0–11.2 mg/dl; phosphorous: 6.1–9.3 mg/dl; sodium: 133.0–159.0 mEq/L; potassium: 3.0–8.2 mEq/L; chloride: 101.4– 143.0 mEq/L; and urea: 11.3–36.8 mg/dl. The red blood cell indices had a narrow range, with mean corpuscular volume: 84.0 102.5 fl and mean corpuscular hemoglobin concentration: 18.2–28.8 g/dl, and which was most likely due to strict physiologic controls. The results from this study were similar to those previously reported for farmed nutria. - Artículo
Acceso Abierto Infección experimental por herpesvirus bovino 1 en conejas gestantes(2011) Valera, Alejandro R.; Pecoraro, Marcelo R.; Fuentealba, N. A.; Massone, Adriana R.; Quiroga, María Alejandra; Corva, Santiago; Galosi, Cecilia MónicaNatural infection with Bovine herpesvirus 1 (BoHV-1) produces several clinical manifestations including conjunctivitis, respiratory signs, genital diseases and abortion. The rabbit is a good model for studying of latency, pathogenicity of BoHV-5 and other bovine herpesviruses. This study was conducted in order to analyze the response to experimental infection with BoHV-1 in rabbits during different periods of pregnancy. The results obtained could be useful for better understanding of the infection in cattle. A new method of infection was used. The rabbits developed clinical signs. The virus was recovered from nasal swabs and histological lesions were found in the analyzed samples. The humoral response was demonstrated and viral DNA was detected from the placentas. This work showed for the first time the arrival of BoHV-1 to blood after intranasal infection with the virus. It also provides a useful tool that can be used to evaluate the pathogenicity of by BoHV-1 strains, the immune response and to study viral latency and reactivation.