Examinando por Autor "Goszczynski, Daniel E."

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  • Artículo
    Acceso Abierto
    Association between GNRHR, LHR and IGF1 polymorphisms and timing of puberty in male Angus cattle
    (2012) Lirón, Juan Pedro; Prando, Alberto; Fernández, María Elena; Ripoli, María Verónica; Rogberg Muñoz, Andrés; Goszczynski, Daniel E.; Baldo, Andrés; Posik, Diego Manuel; Giovambattista, Guillermo; Peral García, Pilar
    Background: In bovines, there are significant differences within and among beef breeds in the time when bulls reach puberty. Although the timing of puberty is likely to be a multigenic trait, previous studies indicate that there may also be single genes that exert major effects on the timing of puberty within the general population. Despite its economic importance, there are not many SNPs or genetic markers associated with the age of puberty in male cattle. In the present work, we selected three candidate genes, GNRHR, LHR and IGF1, and associated their polymorphisms with the age of puberty in Angus male cattle.\nResults: After weaning, 276 Angus males were measured every month for weight (W), scrotal circumference (SC), sperm concentration (C) and percentage of motility (M). A total of 4 SNPs, two within GNRHR, one in LHR and one in IGF1 were genotyped using the pyrosequencing technique. IGF1-SnaBI SNP was significant associated (P < 0.01) with age at SC 28 cm, but it were not associated with age at M 10% and C 50 million. Genotype CC exhibited an average age at SC 28 cm of 7 and 11 days higher than CT (p = 0.037) and TT (p = 0.012), respectively. This SNP explained 1.5% of the genetic variance of age of puberty at SC28. LHR-I499L, GNRHR-SNP5 and GNRHR-SNP6 were not associated with any of the measurements. However, GNRHR haplotypes showed a suggestive association with age at SC 28 cm.\nConclusions: The findings presented here could support the hypothesis that IGF1 is a regulator of the arrival to puberty in male calves and is involved in the events that precede and initiate puberty in bull calves. Given that most studies in cattle, as well as in other mammals, were done in female, the present results are the first evidence of markers associated with age at puberty in male cattle.
  • Artículo
    Acceso Abierto
    La genética molecular de bovinos y equinos criollos en los albores del siglo XXI
    (Sociedad Argentina de Genética, 2010) Giovambattista, Guillermo; Rogberg-Muñoz, Andrés; Ripoli, María Verónica; Villegas-Castagnasso, Egle Etel; Díaz, Silvina; Posik, Diego Manuel; Lirón, Juan Pedro; Carino, Mónica Herminia; Goszczynski, Daniel E.; Fernández, María Elena; Peral García, Pilar
    American Creole bovines and equines are direct descendant from the animals introduced by European conquerors during XV an XVI centuries. At the beginning of the last decade, new technologies based in DNA emerged for genetic analysis, and Creole breeds were not the exception for these kind of studies. During the last years, these methodologies evolved vertiginously. In a short time, the amount of available information increased, and the analysis of few loci turned into analysis of complete genomes. This work describes the evolution of Creole, cattle and equines, molecular genetic studies during the last fifteen years. Even though, Creole breeds have not entered yet into genomic and proteomic era, its study is relevant because they are suffering a progressive population reduction. In this sense, information about infectious diseases resistance, adaptability, forensic genetics, animal production and phylogeography, among others, can be obtained from them. Furthermore, Creole breeds are a unique natural reservoir of genetic variability, and strategies for its conservation should be implemented.