Examinando por Autor "Oliva, Graciela A."

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  • Artículo
    Acceso Abierto
    Diversity of genomic electropherotypes of naturally occurring equine herpesvirus 1 isolates in Argentina
    (1998) Norimine, Junzo; Echeverría, María Gabriela; Oliva, Graciela A.; Nosetto, Edgardo Omar; Etcheverrigaray, María Elisa; Tohya, Y.; Mikami, T.; Galosi, Cecilia Mónica
    The genomes of 10 equine herpcsvirus 1 (EHV-I) strains isolated in Argentina from 1979 to 1991, and a Japanese HH1 reference strain were compared by restriction endonuclease analysis. Two restriction enzymes, BamHI and Bg/II, were used and analysis of the electropherotypes did not show significant differences among isolates obtained from horses with different clinical signs. This suggests that the EHV-1 isolates studied, which circulated in Argentina for more than 10 years, belong to a single genotype.
  • Artículo
    Acceso Abierto
    Infección con el virus de la inmunodeficiencia felina: desarrollo y aplicación de una técnica de doble reacción en cadena de la polimerasa (PRC) para el diagnóstico
    (2000) Oliva, Graciela A.; Vila Roza, M. V.; Teodoroff, T.; Castellano, María Cecilia; Pecoraro, Marcelo R.; Arias, Daniel Osvaldo; González, E. T.; Etcheverrigaray, María Elisa; Galosi, Cecilia Mónica
    A Nested PCR technique for direct identification of Feline Immunodeficiency Virus (FIV) was developed. Two methods for proviral DNA extraction and two pairs of primers were evaluated. The method for amplification was standardized and its specificity was determined by the restriction endonucleases digestion of PCR products. Antibodies specific to FIV were determined using a commercial kit. Thirty heparinized blood samples obtained from cats with and without clinical signs consistent with FIV infection were analyzed. In 17 of the samples antibodies specific to FIV and proviral DNA were detected. The PCR technique proved sensitive and specific. It could be useful in the presence of doubtful serologic results and to detect provirus in cats with recent infections.