Examinando por Autor "Pecoraro, Marcelo R."

Mostrando 1 - 3 de 3
  • Artículo
    Acceso Abierto
    Feline immunodeficiency virus infection: A comparative study of different diagnostic techniques
    (2004) Mórtola, Eduardo Carlos; Oliva, G. A.; Pecoraro, Marcelo R.; Venturini, María Cecilia; Risso, Miguel Atilio
    This study evaluated an indirect immunofluorescence assay (IFA) to detect feline immunodeficiency virus infection (FIV) antibody in a comprehensive epidemiological survey of FIV in Argentina. IFA modified in our laboratory, was compared with two other immunoassays, western blot (WB) and a sandwich immunochromatographic commercial kit (SI), and also with a direct polymerase chain reaction (PCR) method that detects proviral DNA. IFA showed to be a test with high sensitivity and specificity, and could be useful as a diagnostic tool in epidemiological studies. The presence of a low percentage of results with non-specific reactivity in the IFA could be resolved with further testing or use of an alternative method.
  • Artículo
    Acceso Abierto
    Infección con el virus de la inmunodeficiencia felina: desarrollo y aplicación de una técnica de doble reacción en cadena de la polimerasa (PRC) para el diagnóstico
    (2000) Oliva, Graciela A.; Vila Roza, M. V.; Teodoroff, T.; Castellano, María Cecilia; Pecoraro, Marcelo R.; Arias, Daniel Osvaldo; González, E. T.; Etcheverrigaray, María Elisa; Galosi, Cecilia Mónica
    A Nested PCR technique for direct identification of Feline Immunodeficiency Virus (FIV) was developed. Two methods for proviral DNA extraction and two pairs of primers were evaluated. The method for amplification was standardized and its specificity was determined by the restriction endonucleases digestion of PCR products. Antibodies specific to FIV were determined using a commercial kit. Thirty heparinized blood samples obtained from cats with and without clinical signs consistent with FIV infection were analyzed. In 17 of the samples antibodies specific to FIV and proviral DNA were detected. The PCR technique proved sensitive and specific. It could be useful in the presence of doubtful serologic results and to detect provirus in cats with recent infections.
  • Artículo
    Acceso Abierto
    Infección experimental por herpesvirus bovino 1 en conejas gestantes
    (2011) Valera, Alejandro R.; Pecoraro, Marcelo R.; Fuentealba, N. A.; Massone, Adriana R.; Quiroga, María Alejandra; Corva, Santiago; Galosi, Cecilia Mónica
    Natural infection with Bovine herpesvirus 1 (BoHV-1) produces several clinical manifestations including conjunctivitis, respiratory signs, genital diseases and abortion. The rabbit is a good model for studying of latency, pathogenicity of BoHV-5 and other bovine herpesviruses. This study was conducted in order to analyze the response to experimental infection with BoHV-1 in rabbits during different periods of pregnancy. The results obtained could be useful for better understanding of the infection in cattle. A new method of infection was used. The rabbits developed clinical signs. The virus was recovered from nasal swabs and histological lesions were found in the analyzed samples. The humoral response was demonstrated and viral DNA was detected from the placentas. This work showed for the first time the arrival of BoHV-1 to blood after intranasal infection with the virus. It also provides a useful tool that can be used to evaluate the pathogenicity of by BoHV-1 strains, the immune response and to study viral latency and reactivation.