Examinando por Autor "Romero, Jorge Roberto"

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  • Artículo
    Acceso Abierto
    Desarrollo y estandarización de un ELISA indirecto para la detección de anticuerpos contra el Herpesvirus bovino tipo 1
    (1999) Valera, Alejandro Rafael; Galosi, Cecilia Mónica; Echeverría, María Gabriela; Silvestrini, María P.; Romero, Jorge Roberto; Nosetto, Edgardo Omar
    An indirect enzyme linked immunosorbent assay (I-ELISA) was developed to detect antibodies to BHV-1 in serum samples. Nonidet P40-solubilized infected with BHV-I reference strain and mock-infected cell lysates were used as antigens. Serum samples were diluted 1: 64 and a commercial horse radish peroxidase-labelled rabbit anti-bovine lgG was used as second antibody. The reaction was developed using azino-dietilbenzotiazol-sulfonate (ABTS). Cut-off was determined by ratio sample (Rs). Virus neutralization (VN) test was used as a reference test over the 587 samples, 416 of them were positive by VN and 1-EL1SA, 145 were negative by both techniques, and 26 were positive by 1-EL1SA and negative by VN. Using VN as standard, the ELISA showed a relative specificity and sensibility of 100 and 84.79%, respectively.
  • Artículo
    Acceso Abierto
    Efecto del methoprene sobre Musca domestica: ensayos de laboratorio
    (2003) Vignau, María Laura; Romero, Jorge Roberto; Baldo, Andrés; Silvestrini, M. P.; Risso, Miguel Atilio
    To determine methoprene's (MTP) effect on fly Musca domestica emergence were performed laboratory bioassays using a technical formulation identified as MK55, in the form of a granulated insoluble in water. Concentrations of 1.625, 4.5 and 10 ppm of MTP were mixed directly with poultry manure, and larvae I of Musca domestica were fed (test 1). The same MTP concentrations (1.625, 4.5 and 10 ppm) were added to chicken feed and then, the manure produced by the chicken was used as feed to M. domestica larvae I ( test 2); each test involved five assays and one untreated check, in both larvae II and III were grown by feeding manure. Pupae were controlled until fly adults emergence. The percentage of fly growth in each assay decreased in relation to the increase of MTP concentrations and 77.1% of efficacy was shown when 10 ppm was used directly mixed with manure. And 83.7% of efficacy was demonstrated when used as a feed additive. However, a high variability of results was observed in low concentrations. MTP should be used at the highest doses and integrated with other methods to control M. domestica fly in poultry farms.