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Acceso AbiertoActive and pH-Sensitive Nanopackaging Based on Polymeric Anthocyanin/Natural or Organo-Modified Montmorillonite Blends: Characterization and Assessment of Cytotoxicity(2022) Gutiérrez, Tomy J.; León, Ignacio E.; Ponce, Alejandra C.; Álvarez, Vera A.Polymeric anthocyanins are biologically active, pH-sensitive natural compounds and pigments with beneficial functional, pharmacological and therapeutic properties for consumer health. More recently, they have been used for the manufacture of active and pH-sensitive (“intelligent”) food nanopackaging, due to their bathochromic effect. Nevertheless, in order for polymeric anthocyanins to be included either as a functional food or as a pharmacological additive (medicinal food), they inevitably need to be stabilized, as they are highly susceptible to environmental conditions. In this regard, nanopackaging has become a tool to overcome the limitations of polymeric anthocyanins. The objective of this study was to evaluate their structural, thermal, morphological, physicochemical, antioxidant and antimicrobial properties, as well as their responses to pH changes, and the cytotoxicity of blends made from polymeric anthocyanins extracted from Jamaica flowers (Hibiscus sabdariffa) and natural or organo-modified montmorillonite (Mt), as active and pH-sensitive nanopackaging. This study allowed us to conclude that organo-modified Mts are efficient pH-sensitive and antioxidant nanopackaging systems that contain and stabilize polymeric anthocyanins compared to natural Mt nanopackaging and stabilizing polymeric anthocyanins. However, the use of these polymeric anthocyanin-stabilizing organo-modified Mt-based nanopackaging systems are limited for food applications by their toxicity.
Acceso AbiertoCurrent trends on role of biological treatment in integrated treatment technologies of textile wastewater(2021) Ceretta, María Belén; Nercessian, Débora; Wolski, Erika A.Wastewater discharge is a matter of concern as it is the primary source of water pollution. Consequently, wastewater treatment plays a key role in reducing the negative impact that wastewater discharge produce into the environment. Particularly, the effluents produced by textile industry are composed of high concentration of hazardous compounds such as dyes, as well as having high levels of chemical and biological oxygen demand, suspended solids, variable pH, and high concentration of salt. Main efforts have been focused on the development of methods consuming less water or reusing it, and also on the development of dyes with a better fixation capacity. However, the problem of how to treat these harmful effluents is still pending. Different treatment technologies have been developed, such as coagulation-flocculation, adsorption, membrane filtration, reverse osmosis, advanced oxidation, and biological processes (activated sludge, anaerobic-aerobic treatment, and membrane bioreactor). Concerning to biological treatments, even though they are considered as the most environmentally friendly and economic methods, their industrial application is still uncertain. On the one hand, this is due to the costs of treatment plants installation and, on the other, to the fact that most of the studies are carried out with simulated or diluted effluents that do not represent what really happens in the industries. Integrated treatment technologies by combining the efficiency two or more methodologies used to be more efficient for the decontamination of textile wastewater, than treatments used separately. The elimination of hazardous compounds had been reported using combination of physical, chemical, and biological processes. On this way, as degradation products can sometimes be even more toxic than the parent compounds, effluent toxicity assessment is an essential feature in the development of these alternatives. This article provides a critical view on the state of art of biological treatment, the degree of advancement and the prospects for their application, also discussing the concept of integrated treatment and the importance of including toxicity assays to reach an integral approach to wastewater treatment.
Acceso AbiertoEffect of salting procedures on quality of hake (Merluccius hubbsi) fillets(2021) Marchetti, Marion Daniela; Gómez, Paula Luisina; Yeannes, María Isabel; García Loredo, Analía BelénThe influence of salting procedures on the proximate analysis, mechanical parameters, and color of hake (Merluccius hubbsi) was investigated. Three procedures were comparatively evaluated: dry salting (DS), mixed salting (MS) and brining (BS). MS samples had the highest fat content, a considerable protein content and an equilibrium salt content similar to BS. MS samples had a great water loss, as DS method, but hardness and other mechanical parameters were similar to that obtained with BS, i.e. significantly lower than DS. All samples showed color parameters significantly different as compared to fresh hake, turning more red-orange as the salting time increased. Lightness diminished, a* values increased and b* values did not show a clear trend throughout the salting time. Principal component analysis (PCA) described the relationship between some variables (zNaCl, color, and mechanical parameters) with salting time. High Pearson's correlation coefficients were found between zNaCl and hardness, springiness, cohesiveness and a* parameter (r ¼ 0.76, p < 0.001; r ¼ 0.93, p < 0.0001; r ¼ 0.95, p < 0.001 and r ¼ 0.93, p < 0.0001, respectively). Luminosity was negatively correlated with zNaCl (r ¼ -0.87, p ¼ 0.0001). The correlation curves showed nonlinear relationships (R2 adj between 83.7 % and 97.4 %), which could be used to predict quality attributes of hake fillets as a function of salting time. This work contributed to know the effect of different salting procedures on the quality attributes of a species widely available in the Southwest Atlantic Ocean.
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Acceso AbiertoPresentación y líneas de investigación institucionales (2018) Gonzalez, Jorge FroilanEl INCITAA es un Instituto de la Universidad Nacional de Mar del Plata (UNMdP), creado y asociado a la C.I.C. en 2017. Esta presentación resume sus orígenes, las actividades actuales, su producción reciente, actividades en colaboración con otras instituciones y detalla brevemente los trabajos llevados a cabo con apoyo de la C.I.C. a través de dos PIT-AP-BA. El Instituto surgió de la sinergia entre tres grupos de investigación pre-existentes en la Facultad de Ingeniería (F.I.) de la UNMdP: • GIPCAl: (Grupo de Investigación en Procesamiento y Calidad de Alimentos) que inves- tiga sobre el desarrollo y optimización de productos y subproductos a partir de especies marinas mediante el uso y la combinación de diversas barreras biotecnológicas, siendo la estabilidad microbiológica, la obtención de características deseables por el consumi- dor, la extensión de la vida útil y el menor consumo energético en su elaboración, sus principales objetivos deseados. • GIIA: (Grupo de Investigación en Ingeniería de Alimentos) trabaja en técnicas de preser- vación pre y post cosecha de vegetales de bajo impacto ambiental para mantener la cali- dad del producto, su inocuidad y prolongar la vida útil. Las técnicas son las de alimentos mínimamente procesados, la deshidratación de ellos, microencapsulación y extracción de compuestos bioactivos, y las tecnologías de conservación de frutas cortadas y desa- rrollo de jugos con sustancias prebióticas. • GIB: (Grupo de Ingeniería Bioquímica) trabaja en el desarrollo de procesos de biodegra- dación de contaminantes de relevancia local y regional: actualmente la biodegradación de hidrocarburos (un serio problema de contaminación en las aguas del Puerto de Mar del Plata) y la biodegradación de anilinas presentes en el vertido de efluentes de indus- trias locales de teñido, así como el tratamiento de otros efluentes industriales.
Acceso AbiertoTextile dyeing wastewater treatment by Penicillium chrysogenum: Design of a sustainable process(2022) Lanfranconi, Inés; Ceretta, María Belén; Bertola, Nora; Wolski, Erika A.; Durruty, IgnacioIn this work a parametric study and a bench bioreactor degradation test of Direct Black 22 (DB22) by Penicillium chrysogenum was performed as a first approach to an industrial application, framed within a policy of sustainable processes development. Three ancillary carbon sources and their optimum initial concentrations were studied. These were: glucose, potato starch and potato industry wastewater. Their optimum initial concentration was 6 g/L. The use of potato starch as co-substrate showed the highest decolorization rate and COD removal. Degradation of DB22 using different immobilization supports (stainless steel sponge, loofah sponge and polyethylene strips) was studied and the results showed that the time needed for the treatment decreased from 6 to 4 d. Phytotoxicity was evaluated in the final products of the immobilized cells assays, using Lactuca sativa seeds. For all treatments phytoxicity was reduced with respect to the untreated wastewater, except for the assays using polyethylene strips. Finally, the reuse of the biomass attached to different carriers and the performance of the treatment of DB22 in a 1 L bench scale bioreactor were tested. P. chrysogenum decolorized at least four sucesives reuses. The reactor assays showed a better performance of the treatment.
Acceso AbiertoTowards an ideotype for food‑fuel dual‑purpose wheat in Argentina with focus on biogas production(2021) Gabbanelli, Nadia; Erbetta, Elisa; Sanz Smachetti, María Eugenia; Lorenzo, Máximo; Talia, Paola Mónica; Ramírez, Ignacio; Vera, Magdalena; Durruty, Ignacio; Pontaroli, Ana Clara; Echarte, María MercedesBackground: Wheat straw, one of the most abundant agricultural residues worldwide, can be used to produce biogas, which is considered one of the most efficiently produced renewable energies. Wheat grown with the dualpurpose of producing food and biogas should display simultaneously high grain and straw yields, low lodging susceptibility and high conversion efficiency of straw into biogas. The aims of this study were to determine the best food-fuel dual-purpose wheat candidates among 36 wheat genotypes—including French, CIMMYT and local (Criollo) germplasm—used in breeding programs in Argentina and to gain some insights into the relationships between key traits relevant for dual-purpose wheat genotypes. Results: High variability in individual key traits for dual-purpose wheat ideotype has been found. Genotypes of French origin displayed the highest grain yield, and those of CIMMYT origin, the lowest straw yield. Genotypes of Criollo origin showed the highest lodging susceptibility, and French ones, the lowest. Straw yield was positively correlated with grain yield in all genotypes, and negatively correlated with plant height in genotypes of Criollo origin. Straw conversion into biogas was measured in terms of the biogas potential production and kinetic parameters Bmax (maximum specific biogas production) and k (first-order kinetic constant) were analyzed. All key traits were analyzed together by a principal component analysis. Baguette 31 and SNR Nogal, two genotypes of French origin, showed high grain yield, high-to-very high straw yield, low lodging susceptibility, and moderate-to-high Bmax and k. Buck Guapo and Buck Baqueano, two genotypes of Criollo origin, displayed good values for grain yield, straw yield and Bmax and k. However, their high lodging susceptibility precludes their production in shallow soils or high-input systems. Lastly, some old genotypes (e.g. Klein Atlas) harbored a good combination of all key traits and could prove valuable to be included in future breeding programs for dual-purpose wheat. Conclusions: While none of the genotypes excelled in every key trait, a few candidates showed potential for dualpurpose ideotype, particularly Baguette 31 and SNR Nogal. The challenge lays in gathering all attributes for food and 2G fuel in the same genotype.