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dc.date.accessioned 2021-10-07T18:27:38Z
dc.date.available 2021-10-07T18:27:38Z
dc.identifier.uri https://digital.cic.gba.gob.ar/handle/11746/11325
dc.title Effects of fructose-induced metabolic syndrome on rat skeletal cells and tissue, and their responses to metformin treatment en
dc.type Artículo es
dcterms.abstract Aims: Deleterious effects of metabolic syndrome (MS) on bone are still controversial. In this study we evaluated the effects of a fructose-induced MS, and/or an oral treatment with metformin on the osteogenic potential of bone marrow mesenchymal stromal cells (MSC), as well as on bone formation and architecture. Methods: 32 male 8 week-old Wistar rats were assigned to four groups: control (C), control plus oral metformin (CM), rats receiving 10% fructose in drinking water (FRD), and FRD plus metformin (FRDM). Samples were collected to measure blood parameters, and to perform pQCT analysis and static and dynamic histomorphometry. MSC were isolated to determine their osteogenic potential. Results: Metformin improved blood parameters in FRDM rats. pQCTand static and dynamic histomorphometry showed no significant differences in trabecular and cortical bone parameters among groups. FRD reduced TRAP expression and osteocyte density in trabecular bone and metformin only normalized osteocyte density. FRD decreased the osteogenic potential of MSC and metformin administration could revert some of these parameters. Conclusions: FRD-induced MS shows reduction in MSC osteogenic potential, in osteocyte density and in TRAP activity. Oral metformin treatment was able to prevent trabecular osteocyte loss and the reduction in extracellular mineralization induced by FRD-induced MS. en
dcterms.extent 202-213 es
dcterms.issued 2017
dcterms.language Inglés es
dcterms.license Attribution-NonCommercial-ShareAlike 4.0 International (BY-NC-SA 4.0) es
dcterms.subject Metabolic syndrome en
dcterms.subject Fructose en
dcterms.subject Bone architecture en
dcterms.subject Osteoblasts en
dcterms.subject Metformin en
cic.version info:eu-repo/semantics/publishedVersion es
dcterms.creator.author Felice, Juan Ignacio es
dcterms.creator.author Schurman, León es
dcterms.creator.author McCarthy, Antonio Desmond es
dcterms.creator.author Sedlinsky, Claudia es
dcterms.creator.author Aguirre, José Ignacio es
dcterms.creator.author Cortizo, Ana María es
cic.lugarDesarrollo Laboratorio de Investigación en Osteopatías y Metabolismo Mineral es
dcterms.subject.materia Ciencias Biológicas es
dcterms.identifier.other doi:10.1016/j.diabres.2017.02.011 es
dcterms.identifier.other ISSN:0168-8227 es
dcterms.isPartOf.issue vol. 126 es
dcterms.isPartOf.series Diabetes Research and Clinical Practice es
cic.isPeerReviewed true es
cic.isFulltext true es
cic.institucionOrigen Laboratorio de Investigación en Osteopatías y Metabolismo Mineral es


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