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dc.title Insulin-mimetic action of vanadium compounds on osteoblast-like cells in culture en
dc.type Artículo es
dcterms.abstract Vanadium compounds mimic insulin actions in different cell types. The present study concerns the insulin-like effects of three vanadium(V) derivatives and one vanadium(IV) complex on osteoblast-like (UMR106 and MC3T3E1) cells in culture. The vanadium oxalate and vanadium citrate complexes hydrolyzed completely under the culture conditions, whereas more than 40% of the vanadium tartrate and nitrilotriacetate complexes remained. Vanadate, as well as vanadium oxalate, citrate, and tartrate complexes enhanced cell proliferation (as measured by the crystal violet assay), glucose consumption, and protein content in UMR106 and MC3T3E1 osteoblast-like cells. The vanadium nitrilotriacetate complex (the only peroxo complex tested) stimulated cell proliferation in UMR106 but not in MC3T3E1 cells. This derivative strongly transformed the morphology of the MC3T3E1 cells. All vanadium(V) compounds inhibited cell differentiation (alkaline phosphatase activity) in UMR106 cells. Our data are consistent with the interpretation that vanadium oxalate and citrate complexes hydrolyze to vanadate. Vanadium nitrilotriacetate would appear to be toxic for normal MC3T3E1 osteoblasts. In contrast, the vanadium tartrate complex induced a proliferative effect; however, it did not alter cell differentiation. en
dcterms.extent 8 p. es
dcterms.issued 1997
dcterms.language Inglés es
dcterms.license Attribution 4.0 International (BY 4.0) es
dcterms.subject vanadate en
dcterms.subject aqueous vanadium chemistry en
dcterms.subject Vanastability en
dcterms.subject spectroscopy en
dcterms.subject proliferation en
dcterms.subject differentiation en
dcterms.subject glucose consumption en
dcterms.subject protein content en
dcterms.subject alkaline phosphatase en
dcterms.subject osteoblast-like cells en
cic.version info:eu-repo/semantics/submittedVersion es Etcheverry, Susana B. es Crans, D. C. es Keramidas, A. D. es Cortizo, Ana María es
cic.lugarDesarrollo Universidad Nacional de La Plata es
dcterms.subject.materia Ciencias Químicas es
dcterms.isPartOf.issue vol. 338, no. 1 es
dcterms.isPartOf.series Archives of Biochemistry and Biophysics es
cic.isPeerReviewed true es
cic.isFulltext true es


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