Show simple item record 2017-09-15T17:37:08Z 2017-09-15T17:37:08Z
dc.title ToxGen: an improved reference database for the identification of type B-trichothecene genotypes in Fusarium en
dc.type Artículo es
dcterms.abstract Type B trichothecenes, which pose a serious hazard to consumer health, occur worldwide in grains. These mycotoxins are produced mainly by three different trichothecene genotypes/chemotypes: 3ADON (3-acetyldeoxynivalenol), 15ADON (15- acetyldeoxynivalenol) and NIV (nivalenol), named after these three major mycotoxin compounds. Correct identification of these genotypes is elementary for all studies relating to population surveys, fungal ecology and mycotoxicology. Trichothecene producers exhibit enormous strain-dependent chemical diversity, which may result in variation in levels of the genotype’s determining toxin and in the production of low to high amounts of atypical compounds. New high-throughput DNA-sequencing technologies promise to boost the diagnostics of mycotoxin genotypes. However, this requires a reference database containing a satisfactory taxonomic sampling of sequences showing high correlation to actually produced chemotypes. We believe that one of the most pressing current challenges of such a database is the linking of molecular identification with chemical diversity of the strains, as well as other metadata. In this study, we use the Tri12 gene involved in mycotoxin biosynthesis for identification of Tri genotypes through sequence comparison. Tri12 sequences from a range of geographically diverse fungal strains comprising 22 Fusarium species were stored in the ToxGen database, which covers descriptive and up-to-date annotations such as indication on Tri genotype and chemotype of the strains, chemical diversity, information on trichothecene-inducing host, substrate or media, geographical locality, and most recent taxonomic affiliations. The present initiative bridges the gap between the demands of comprehensive studies on trichothecene producers and the existing nucleotide sequence databases, which lack toxicological and other auxiliary data. We invite researchers working in the fields of fungal taxonomy, epidemiology and mycotoxicology to join the freely available annotation effort. en
dcterms.extent 19 p. es
dcterms.issued 2017
dcterms.language Inglés es
dcterms.license Attribution 4.0 International (BY 4.0) es
dcterms.subject Bioinformatics en
dcterms.subject Mycology en
dcterms.subject Trichothecene genotypes en
dcterms.subject Fusarium es
dcterms.subject Chemotypes en
dcterms.subject Molecular identification en
dcterms.subject Annotation en
cic.version info:eu-repo/semantics/publishedVersion es Kulik, Tomasz es Abarenkov, Kessy es Buśko, Maciej es Bilska, Katarzyna es Diepeningen, Anne D. van es Ostrowska-Kołodziejczak, Anna es Krawczyk, Katarzyna es Brankovics, Balázs es Stenglein, Sebastián es Sawicki, Jakub es Perkowski, Juliusz es
cic.lugarDesarrollo Laboratorio de Biología Funcional y Biotecnología es
dcterms.subject.materia Ciencias de las Plantas, Botánica es
dcterms.identifier.url Recurso completo es
dcterms.identifier.other DOI: 10.7717/peerj.2992 es
dcterms.isPartOf.issue 5 es
dcterms.isPartOf.series PeerJ es
cic.isPeerReviewed true es
cic.isFulltext true es


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