Show simple item record 2018-03-22T15:47:32Z 2018-03-22T15:47:32Z
dc.title Biochemical and MALDI-TOF Mass Spectrometric Characterization of a Novel Native and Recombinant Cystine Knot Miniprotein from Solanum tuberosum subsp. andigenum cv. Churqueña en
dc.type Artículo es
dcterms.abstract Cystine-knot miniproteins (CKMPs) are an intriguing group of cysteine-rich molecules that combine the characteristics of proteins and peptides. Typically, CKMPs are fewer than 50 residues in length and share a characteristic knotted scaffold characterized by the presence of three intramolecular disulfide bonds that form the singular knotted structure. The knot scaffold confers on these proteins remarkable chemical, thermal, and proteolytic stability. Recently, CKMPs have emerged as a novel class of natural molecules with interesting pharmacological properties. In the present work, a novel cystine-knot metallocarboxypeptidase inhibitor (chuPCI) was isolated from tubers of Solanum tuberosum, subsp. andigenum cv. Churqueña. Our results demonstrated that chuPCI is a member of the A/B-type family of metallocarboxypeptidases inhibitors. chuPCI was expressed and characterized by a combination of biochemical and mass spectrometric techniques. Direct comparison of the MALDI-TOF mass spectra for the native and recombinant molecules allowed us to confirm the presence of four different forms of chuPCI in the tubers. The majority of such forms have a molecular weight of 4309 Da and contain a cyclized Gln in the N-terminus. The other three forms are derived from N-terminal and/or C-terminal proteolytic cleavages. Taken together, our results contribute to increase the current repertoire of natural CKMPs. en
dcterms.extent 19 p. es
dcterms.issued 2018-03
dcterms.language Inglés es
dcterms.license Attribution 4.0 International (BY 4.0) es
dcterms.publisher MDPI (Multidisciplinary Digital Publishing Institute) es
dcterms.subject cystine-knot miniproteins en
dcterms.subject carboxypeptidase inhibitor en
dcterms.subject plant inhibitor en
dcterms.subject Solanum tuberosum es
dcterms.subject protease en
dcterms.subject Andean potatoes en
cic.version info:eu-repo/semantics/publishedVersion es Cotabarren, Juliana es Tellechea, Mariana Edith es Tanco, Sebastián Martín es Lorenzo, Julia es Garcia-Pardo, Javier es Avilés, Francesc Xavier es Obregón, Walter David es
cic.lugarDesarrollo Centro de Investigación de Proteínas Vegetales es
dcterms.subject.materia Bioquímica y Biología Molecular es
dcterms.identifier.url Recurso online es
dcterms.identifier.other DOI :10.3390/ijms19030678 es
dcterms.isPartOf.issue vol. 19, no. 3 es
dcterms.isPartOf.series International Journal of Molecular Sciences es
cic.isPeerReviewed true es
cic.isFulltext true es


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