Show simple item record 2018-04-20T14:37:46Z 2018-04-20T14:37:46Z
dc.title Draft Genome Sequence of the Plant-Pathogenic Fungus Stemphylium 2 lycopersici Strain CIDEFI-216 en
dc.type Documento de conferencia es
dcterms.abstract <em>Stemphylium lycopersici</em>is a pathogenic fungus that provokes the leaf spot disease on over 30 host genera worldwide, among them Tomato. Here we report the draft genome sequence of<em>S. lycopersici</em>strain CIDEFI-216. Total genomic DNA was isolated from a monosporic culture using the DNeasy Plant Mini Kit (Qiagen) and was used to construct 100 bp paired-end libraries, which were then sequenced by Illumina HiSeq 2000 technology. Reads were assembled using SOAPdenovo2 software at Macrogen (Korea).Gene prediction was performed by Fgenesh software (Softberry), functional annotation was carried out with Blast2GO software (BioBam) and tRNAs and rRNAs were predicted usingtRNAscan-SE and HMM-rRNA tools from WebMGA server. The paired-end libraries produced 31117554 reads with a total of 3142872954 bp, representing an average coverage of 77.39 X. The genome was assembled into 419 scaffolds with a total length of 35.18 Mbp (1000 bp; N50=498048 bp) and an overall G+C content of 50.5 %. A total of 8998 protein-coding genes were predicted, whose functional annotation is discussed. Additionally, 94 tRNAs and 44 rRNAs were found. This draft genome sequence, the first available for<em>S. lycopersici</em>, represents a new resource for further research into the taxonomy, biology and phytopathology of this plant pathogen. en
dcterms.extent 2 p. es
dcterms.issued 2015-08-05
dcterms.language Inglés es
dcterms.license Attribution-NonCommercial-NoDerivatives 4.0 International (BY-NC-ND 4.0) es
dcterms.subject Draft genome en
dcterms.subject Stemphylium lycopersici en
dcterms.subject Gene Annotation en
cic.version info:eu-repo/semantics/submittedVersion es Franco, Mario Emilio Ernesto es López, Silvina Marianela Yanil es Medina, Rocío es Saparrat, Mario es Balatti, Pedro Alberto es
cic.lugarDesarrollo Instituto de Fisiología Vegetal es
cic.lugarDesarrollo Centro de Investigaciones de Fitopatología es
dcterms.subject.materia Agronomía, reproducción y protección de plantas es
dcterms.isPartOf.issue APS Annual meeting Crossroad in science (Pasadena, 2015) es
dcterms.isPartOf.series APS Annual meeting Crossroad in science es
cic.isPeerReviewed true es
cic.isFulltext true es


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