Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat
| cic.isFulltext | true | es |
| cic.isPeerReviewed | true | es |
| cic.lugarDesarrollo | Laboratorio de Biología Funcional y Biotecnología | es |
| cic.version | info:eu-repo/semantics/submittedVersion | es |
| dc.date.accessioned | 2018-04-11T16:02:17Z | |
| dc.date.available | 2018-04-11T16:02:17Z | |
| dc.identifier.uri | https://digital.cic.gba.gob.ar/handle/11746/7137 | |
| dc.title | Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat | es |
| dc.type | Artículo | es |
| dcterms.abstract | <strong>Background</strong> Low-molecular-weight glutenin subunits (LMW-GS) play a crucial role in determining end-use quality of common wheat by influencing the viscoelastic properties of dough. Four different methods - sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), two-dimensional gel electrophoresis (2-DE, IEF × SDS-PAGE), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and polymerase chain reaction (PCR), were used to characterize the LMW-GS composition in 103 cultivars from 12 countries. <strong>Results</strong> At the<em>Glu-A3</em>locus, all seven alleles could be reliably identified by 2-DE and PCR. However, the alleles<em>Glu-A3e</em>and<em>Glu-A3d</em>could not be routinely distinguished from<em>Glu-A3f</em>and<em>Glu-A3g</em>, respectively, based on SDS-PAGE, and the allele<em>Glu-A3a</em>could not be differentiated from<em>Glu-A3c</em>by MALDI-TOF-MS. At the<em>Glu-B3</em>locus, alleles<em>Glu-B3a</em>,<em>Glu-B3b</em>,<em>Glu-B3c</em>,<em>Glu-B3g</em>,<em>Glu-B3h</em>and<em>Glu-B3j</em>could be clearly identified by all four methods, whereas<em>Glu-B3ab</em>,<em>Glu-B3ac</em>,<em>Glu-B3ad</em>could only be identified by the 2-DE method. At the<em>Glu-D3</em>locus, allelic identification was problematic for the electrophoresis based methods and PCR. MALDI-TOF-MS has the potential to reliably identify the<em>Glu-D3</em>alleles. <strong>Conclusions</strong> PCR is the simplest, most accurate, lowest cost, and therefore recommended method for identification of<em>Glu-A3</em>and<em>Glu-B3</em>alleles in breeding programs. A combination of methods was required to identify certain alleles, and would be especially useful when characterizing new alleles. A standard set of 30 cultivars for use in future studies was chosen to represent all LMW-GS allelic variants in the collection. Among them, Chinese Spring, Opata 85, Seri 82 and Pavon 76 were recommended as a core set for use in SDS-PAGE gels.<em>Glu-D3c</em>and<em>Glu-D3e</em>are the same allele. Two new alleles, namely,<em>Glu-D3m</em>in cultivar Darius, and<em>Glu-D3n</em>in Fengmai 27, were identified by 2-DE. Utilization of the suggested standard cultivar set, seed of which is available from the CIMMYT and INRA Clermont-Ferrand germplasm collections, should also promote information sharing in the identification of individual LMW-GS and thus provide useful information for quality improvement in common wheat. | en |
| dcterms.creator.author | Liu, Li | es |
| dcterms.creator.author | Ikeda, Tatsuya M. | es |
| dcterms.creator.author | Branlard, Gerard | es |
| dcterms.creator.author | Peña, Roberto J. | es |
| dcterms.creator.author | Rogers, John William | es |
| dcterms.creator.author | Lerner, Silvia E. | es |
| dcterms.creator.author | Kolman, Maía de Los Angeles | es |
| dcterms.creator.author | Xia, Xianchun | es |
| dcterms.creator.author | Wang, Linhai | es |
| dcterms.creator.author | Ma, Wujun | es |
| dcterms.creator.author | Appels, Rudi | es |
| dcterms.creator.author | Yoshida, Hisashi | es |
| dcterms.creator.author | Wang, Aili | es |
| dcterms.creator.author | Yan, Yueming | es |
| dcterms.creator.author | He, Zhonghu | es |
| dcterms.extent | 24 p. | es |
| dcterms.identifier.other | doi:10.1186/1471-2229-10-124 | es |
| dcterms.identifier.url | Recurso Completo | es |
| dcterms.isPartOf.issue | vol. 210 | es |
| dcterms.isPartOf.series | BMC Plant Biology | es |
| dcterms.issued | 2010 | |
| dcterms.language | Inglés | es |
| dcterms.license | Attribution 4.0 International (BY 4.0) | es |
| dcterms.subject | Common Wheat | en |
| dcterms.subject | Glutenin Subunit | en |
| dcterms.subject | Standard Cultivar | en |
| dcterms.subject | Glutenin Protein | en |
| dcterms.subject | Glutenin Extract | en |
| dcterms.subject.materia | Agronomía, reproducción y protección de plantas | es |
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