Video processing analysis for the determination and evaluation of the chemotactic response in bacterial populations

cic.isFulltexttruees
cic.isPeerReviewedtruees
cic.lugarDesarrolloUniversidad Nacional de Mar del Plataes
cic.versioninfo:eu-repo/semantics/submittedVersiones
dc.date.accessioned2020-08-05T11:43:31Z
dc.date.available2020-08-05T11:43:31Z
dc.identifier.urihttps://digital.cic.gba.gob.ar/handle/11746/10643
dc.titleVideo processing analysis for the determination and evaluation of the chemotactic response in bacterial populationsen
dc.typeArtículoes
dcterms.abstractThe aim of the present work was to design a methodology based on video processing to obtain indicators of bacterial population motility that allow the quantitative and qualitative analysis and comparison of the chemotactic phenomenon with different attractants in the agarose-in plug bridge method. Video image sequences were processed applying Shannon's entropy to the intensity time series of each pixel, which conducted to a final pseudo colored image resembling a map of the dynamic bacterial clusters. Processed images could discriminate perfectly between positive and negative attractant responses at different periods of time from the beginning of the assay. An index of spatial and temporal motility was proposed to quantify the bacterial response. With this index, this video processing method allowed obtaining quantitative information of the dynamic changes in space and time from a traditional qualitative assay. We conclude that this computational technique, applied to the traditional agarose-in plug assay, has demonstrated good sensitivity for identifying chemotactic regions with a broad range of motility.en
dcterms.creator.authorNisenbaum, Melinaes
dcterms.creator.authorMaldonado, Emilioes
dcterms.creator.authorMartinez Arca, Jorgees
dcterms.creator.authorGonzalez, Jorge Froilanes
dcterms.creator.authorPassoni, Lucía I.es
dcterms.creator.authorMurialdo, Silvia Elenaes
dcterms.identifier.urlRecurso completoes
dcterms.isPartOf.issuevol. 127es
dcterms.isPartOf.seriesJournal of Microbiological Methodses
dcterms.issued2016
dcterms.languageIngléses
dcterms.licenseAtribución-NoComercial 4.0 Internacionales
dcterms.subjectvideoes
dcterms.subjectQuimiotaxises
dcterms.subject.materiaCiencias Biológicases
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