PLPKI: A novel serine protease inhibitor as a potential biochemical marker involved in horizontal resistance to Phytophthora infestans

cic.isFulltexttruees
cic.isPeerReviewedtruees
cic.lugarDesarrolloUniversidad Nacional de Mar del Plata es
cic.versioninfo:eu-repo/semantics/submittedVersiones
dc.date.accessioned2017-04-06T12:13:20Z
dc.date.available2017-04-06T12:13:20Z
dc.identifier.urihttps://digital.cic.gba.gob.ar/handle/11746/5524
dc.titlePLPKI: A novel serine protease inhibitor as a potential biochemical marker involved in horizontal resistance to Phytophthora infestansen
dc.typeArtículoes
dcterms.abstractPotato leaves infected with <em>Phytophthorainfestans</em> produced a serine protease inhibitor (PLPKI) with specificity for microbial proteases. Sequencing of the first twenty residues at the NH2-terminus of the mature PLPKI polypeptide demonstrated that PLPKI is a novel member of the potato protease inhibitor I family. PLPKI inhibited the activity of extracellular proteases produced by two pathogens of potato, <em>P.infestans</em> and <em>Rhizoctoniasolani</em>, but was inactive against proteases secreted into the culture media by the binucleate <em>Rhizoctonia</em> N2, a non-pathogenic fungus for potato. Western blot analysis showed a positive correlation between the levels of PLPKI and the degree of horizontal resistance, showing its highest accumulation in clone OKA 5632.11, which has been described as highly resistant. This correlation, together with the ability of PLPKI to completely abolish the secreted serine protease activity of <em>P.infestans</em>, suggests that PLPKI may have an active role in protecting potato plants from this pathogenic oomycete and that it could be used as a suitable biochemical marker to help breeders in the selection of cultivars with high degree of horizontal resistance.en
dcterms.creator.authorFeldman, M.es
dcterms.creator.authorAndreu, A.B.es
dcterms.creator.authorKorgan, S.es
dcterms.creator.authorLobato, M.C.es
dcterms.creator.authorHuarte, M.es
dcterms.creator.authorLee Walling, Lindaes
dcterms.creator.authorDaleo, Gustavo Raúles
dcterms.extent6 p.es
dcterms.identifier.other10.1111/pbr.12129es
dcterms.isPartOf.issuevol. 133, no. 2es
dcterms.isPartOf.seriesPlant Breedinges
dcterms.issued2014-01-06
dcterms.languageIngléses
dcterms.licenseAttribution-NonCommercial-NoDerivatives 4.0 International (BY-NC-ND 4.0)es
dcterms.subjectplant defenceen
dcterms.subjectPotatoen
dcterms.subjectquantitative resistanceen
dcterms.subject.materiaBioquímica y Biología Moleculares
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