SRAP as an Informative Molecular Marker to Study the Fusarium poae Genetic Variability

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cic.lugarDesarrolloLaboratorio de Biología Funcional y Biotecnología es
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dc.date.accessioned2017-10-25T12:56:56Z
dc.date.available2017-10-25T12:56:56Z
dc.identifier.urihttps://digital.cic.gba.gob.ar/handle/11746/6292
dc.titleSRAP as an Informative Molecular Marker to Study the Fusarium poae Genetic Variabilityen
dc.typeArtículoes
dcterms.abstract<em>Fusarium poae</em>is one of the<em>Fusarium</em>species isolated from grains associated with Fusarium head blight (FHB), whose occurrence has increased in the last years. In this study, a total of 105<em>F.poae</em>isolates from Argentina, Belgium, Canada, England, Finland, France, Germany, Hungary, Italy, Luxembourg, Poland, Switzerland and Uruguay were evaluated using sequence-related amplified polymorphism (SRAP) to analyse the capacity of this molecular marker to evaluate the<em>F.poae</em>genetic variability. The molecular analysis showed high intraspecific variability within<em>F.poae</em>isolates, and a partial relationship was revealed between variability and the host/geographic origin. Analysis of molecular variance (amova) indicated a high genetic variability in the<em>F.poae</em>collection, with most of the genetic variability resulting from differences within, rather than between American and European populations. The analysis of sequenced SRAP fragments targets into hypothetical proteins from different<em>Fusarium</em>species showing that the SRAP technique not only allows studying<em>F.poae</em>genetic variability, but also targets coding regions into the<em>F.poae</em>genome. To our knowledge, this is the first report on genetic variability of<em>F.poae</em>using SRAP technique and also demonstrates the efficacy of this molecular marker to amplify open reading frames in fungus.es
dcterms.creator.authorDinolfo, María Inéses
dcterms.creator.authorCastañares, Elianaes
dcterms.creator.authorStenglein, Sebastiánes
dcterms.extent7 p.es
dcterms.isPartOf.issuevol. 163es
dcterms.isPartOf.seriesJournal of Phytopathologyes
dcterms.issued2015
dcterms.languageIngléses
dcterms.licenseAttribution-NonCommercial 4.0 International (BY-NC 4.0)es
dcterms.subjectFusarium poaeen
dcterms.subjectgenetic variabilityen
dcterms.subjectopen reading framesen
dcterms.subjectsequence-related amplified polymorphismen
dcterms.subject.materiaAgronomía, reproducción y protección de plantases

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