Ghrelin Indirectly Activates Hypophysiotropic CRF Neurons in Rodents

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cic.lugarDesarrolloInstituto Multidisciplinario de Biología Celular es
cic.versioninfo:eu-repo/semantics/publishedVersiones
dc.date.accessioned2016-08-25T14:14:16Z
dc.date.available2016-08-25T14:14:16Z
dc.identifier.urihttps://digital.cic.gba.gob.ar/handle/11746/4014
dc.titleGhrelin Indirectly Activates Hypophysiotropic CRF Neurons in Rodentsen
dc.typeArtículoes
dcterms.abstractGhrelin is a stomach-derived hormone that regulates food intake and neuroendocrine function by acting on its receptor, GHSR (Growth Hormone Secretagogue Receptor). Recent evidence indicates that a key function of ghrelin is to signal stress to the brain. It has been suggested that one of the potential stress-related ghrelin targets is the CRF (Corticotropin-Releasing Factor)-producing neurons of the hypothalamic paraventricular nucleus, which secrete the CRF neuropeptide into the median eminence and activate the hypothalamic-pituitary-adrenal axis. However, the neural circuits that mediate the ghrelin-induced activation of this neuroendocrine axis are mostly uncharacterized. In the current study, we characterized in vivo the mechanism by which ghrelin activates the hypophysiotropic CRF neurons in mice. We found that peripheral or intra-cerebro-ventricular administration of ghrelin strongly activates c-fos – a marker of cellular activation – in CRFproducing neurons. Also, ghrelin activates CRF gene expression in the paraventricular nucleus of the hypothalamus and the hypothalamic-pituitary-adrenal axis at peripheral level. Ghrelin administration directly into the paraventricular nucleus of the hypothalamus also induces c-fos within the CRF-producing neurons and the hypothalamic-pituitary-adrenal axis, without any significant effect on the food intake. Interestingly, dual-label immunohistochemical analysis and ghrelin binding studies failed to show GHSR expression in CRF neurons. Thus, we conclude that ghrelin activates hypophysiotropic CRF neurons, albeit indirectly.en
dcterms.creator.authorCabral, Agustinaes
dcterms.creator.authorSuescun, Olgaes
dcterms.creator.authorZigman , J.M.es
dcterms.creator.authorPerelló, Marioes
dcterms.extent10 p.es
dcterms.identifier.otherhttp://dx.doi.org/10.1371/journal.pone.0031462es
dcterms.identifier.urlDocumento completoes
dcterms.isPartOf.issuevol. 7, nº 2es
dcterms.isPartOf.seriesPLoS ONEes
dcterms.issued2012-02
dcterms.languageIngléses
dcterms.licenseAttribution 4.0 International (BY 4.0)es
dcterms.subjectGhrelinaes
dcterms.subjectNeuronases
dcterms.subjectEstrés Psicológicoes
dcterms.subject.materiaBiología Celular, Microbiologíaes
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