First evidence of chromosomal variation within Chelonoidis chilensis (Testudines: Testudinidae)

cic.isFulltexttruees
cic.isPeerReviewedtruees
cic.lugarDesarrolloInstituto Multidisciplinario de Biología Celular es
cic.versioninfo:eu-repo/semantics/submittedVersiones
dc.date.accessioned2016-09-09T19:13:58Z
dc.date.available2016-09-09T19:13:58Z
dc.identifier.urihttps://digital.cic.gba.gob.ar/handle/11746/4208
dc.titleFirst evidence of chromosomal variation within Chelonoidis chilensis (Testudines: Testudinidae)es
dc.typeArtículoes
dcterms.abstract<i>Chelonoidis chilensis</i> is an endangered tortoise that inhabits arid regions in Argentina, Bolivia and Paraguay. Blood samples were obtained from wild specimens from the Argentinan distribution range together with samples from specimens of known morphotype but unknown provenance. Cytogenetic analysis using Giemsa staining showed that the diploid chromosome complement was 2n=52 for all twenty-five tortoises analysed. Two different karyomorphs, termed A and B, were identified, with a karyotypic formulae of 7:5:14 and 6:5:15, respectively. G-band analysis suggests that karyomorph B may originate from a chromosomal fission event involving chromosome pair 7 of karyomorph A. In addition, all specimens analysed using Fluorescence In Situ Hybridisation (FISH) with a telomeric probe showed telomeric signals only at the terminal regions of chromosomes. This evidence suggests that the karyotype of <i>C. chilensis</i> does not have telocentric chromosomes, and that interstitial telomeric sequences have not played a major role during the recent chromosomal evolution of this species. Our data agree with recent molecular evidence supporting the existence of one instead several species for the <i>C. chilensis</i> complex. Our data further suggest a possible correlation between chromosomal variation and geographical distribution: karyomorph A is present in tortoises from the Dry Chaco Eco-region, whereas karyomorph B characterises tortoises living in the Monte of Steps and Plains Eco-region. Morphology appears to vary independently of cytomorph variation.es
dcterms.creator.authorSánchez, J.es
dcterms.creator.authorAlcalde, L.es
dcterms.creator.authorBolzan, Agustín Eduardoes
dcterms.extentp. 83-89es
dcterms.isPartOf.issuevol. 25es
dcterms.isPartOf.seriesHerpetological Journales
dcterms.issued2015
dcterms.languageIngléses
dcterms.licenseAttribution 4.0 International (BY 4.0)es
dcterms.subjectChelonoidis chilensises
dcterms.subjectcryptodiraes
dcterms.subjectFluorescence In Situ hybridisation (FISH)en
dcterms.subjectkaryotypic evolutionen
dcterms.subjecttestudinesen
dcterms.subject.materiaZoología, Ornitología, Entomología, Etologíaes

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