ISSR markers detect high genetic variation among Fusarium poae isolates from Argentina and England

cic.isFulltexttruees
cic.isPeerReviewedtruees
cic.lugarDesarrolloLaboratorio de Biología Funcional y Biotecnología es
cic.versioninfo:eu-repo/semantics/submittedVersiones
dc.date.accessioned2018-03-27T17:04:27Z
dc.date.available2018-03-27T17:04:27Z
dc.identifier.urihttps://digital.cic.gba.gob.ar/handle/11746/7074
dc.titleISSR markers detect high genetic variation among Fusarium poae isolates from Argentina and Englanden
dc.typeArtículoes
dcterms.abstractFusarium poae is one of the Fusarium species isolated from cereal grains infected by Fusarium head blight (FHB), and in recent years it has been identified as a major FHB component. In this study, 97 F. poae isolates from Argentina (n=62) and England (n=35) were analysed by inter-simple sequence repeats (ISSR) to examine the genetic diversity and to determine whether intraspecific variation could be correlated with geographic and/or host origin. The molecular analysis showed high intraspecific variability within F. poae isolates, but did not reveal a clear relationship between variability and the host/geographic origin. Fusarium poae isolates from the same geographic region or host appeared in different subclusters. Conversely, isolates with the same haplotype were also collected from different geographic regions. However, we did observe subclusters consisting of isolates from Argentina only or from England only. Furthermore, a single seed sample was found to host different haplotypes. Analysis of molecular variance (AMOVA) indicated a high genetic variability in F. poae, with most of the genetic variability explained by differences within, rather than between Argentinean and English populations. This is the first report on genetic diversity of F. poae using ISSR markers. Moreover, ISSR fingerprinting generates highly polymorphic markers for F. poae and proved to be a useful and reliable assay for genetic variability studies.en
dcterms.creator.authorDinolfo, María Inéses
dcterms.creator.authorStenglein, Sebastiánes
dcterms.creator.authorMoreno, María Virginiaes
dcterms.creator.authorNicholson, Paules
dcterms.creator.authorJennings, Philipes
dcterms.creator.authorSalerno, Graciela L.es
dcterms.extentp. 483–491es
dcterms.identifier.otherDOI 10.1007/s10658-010-9613-1es
dcterms.identifier.urlRecurso Completoes
dcterms.isPartOf.issuevol. 127es
dcterms.isPartOf.seriesEuropean Journal of Plant Pathologyes
dcterms.issued2010
dcterms.languageIngléses
dcterms.licenseAttribution 4.0 International (BY 4.0)es
dcterms.subjectFusarium poaeen
dcterms.subjectGenetic variabilityen
dcterms.subjectInter-simple sequence repeatsen
dcterms.subject.materiaCiencias Biológicases

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