Heteroalleles in Common Wheat
| cic.institucionOrigen | Laboratorio de Biología Funcional y Biotecnología | |
| cic.isFulltext | SI | |
| cic.isPeerReviewed | true | |
| cic.lugarDesarrollo | Laboratorio de Biología Funcional y Biotecnología | |
| cic.parentType | Artículo | |
| cic.version | Publicada | |
| dc.date.accessioned | 2023-06-26T18:18:38Z | |
| dc.date.available | 2023-06-26T18:18:38Z | |
| dc.identifier.uri | https://digital.cic.gba.gob.ar/handle/11746/11964 | |
| dc.title | Heteroalleles in Common Wheat | en |
| dc.type | Artículo | |
| dcterms.abstract | The Gli-B1-encoded -gliadins and non-coding -gliadin DNA sequences for 15 different alleles of common wheat have been compared using seven tests: electrophoretic mobility (EM) and molecular weight (MW) of the encoded major -gliadin, restriction fragment length polymorphism patterns (RFLPs) (three different markers), Gli-B1- -gliadin-pseudogene known SNP markers (Single nucleotide polymorphisms) and sequencing the pseudogene GAG56B. It was discovered that encoded -gliadins, with contrasting EM, had similar MWs. However, seven allelic variants (designated from I to VII) differed among them in the other six tests: I (alleles Gli-B1i, k, m, o), II (Gli-B1n, q, s), III (Gli-B1b), IV (Gli-B1e, f, g), V (Gli-B1h), VI (Gli-B1d) and VII (Gli-B1a). Allele Gli-B1c (variant VIII) was identical to the alleles from group IV in four of the tests. Some tests might show a fine difference between alleles belonging to the same variant. Our results attest in favor of the independent origin of at least seven variants at the Gli-B1 locus that might originate from deeply diverged genotypes of the donor(s) of the B genome in hexaploid wheat and therefore might be called “heteroallelic”. The donor’s particularities at the Gli-B1 locus might be conserved since that time and decisively contribute to the current high genetic diversity of common wheat. | en |
| dcterms.creator.author | Metakovsky, Eugene | |
| dcterms.creator.author | Pascual, Laura | |
| dcterms.creator.author | Vaccino, Patrizia | |
| dcterms.creator.author | Melnik, Viktor | |
| dcterms.creator.author | Rodriguez Quijano, Marta | |
| dcterms.creator.author | Popovych, Yulia | |
| dcterms.creator.author | Chebotar, Sabina | |
| dcterms.creator.author | Rogers, John William | |
| dcterms.identifier.other | DOI:10.3390/ijms22041832 | |
| dcterms.identifier.url | https://www.mdpi.com/1422-0067/22/4/1832 | |
| dcterms.isPartOf.issue | vol. 22, no. 4 | |
| dcterms.isPartOf.series | International Journal of Molecular Sciences | |
| dcterms.issued | 2021-02-12 | |
| dcterms.language | Inglés | |
| dcterms.license | Attribution 4.0 International (BY 4.0) | |
| dcterms.subject | Triticum aestivum | en |
| dcterms.subject | Gli-B1 | en |
| dcterms.subject | γ-gliadin polymorphism | en |
| dcterms.subject | APAGE | en |
| dcterms.subject | PCR | en |
| dcterms.subject | DNA sequencing | en |
| dcterms.subject.materia | Ciencias Biológicas | |
| dcterms.title.subtitle | Multiple Differences between Allelic Variants of the Gli-B1 Locus | en |