Calcium-dependent Protein Kinases are Involved in Potato Signal Transduction.
| cic.isFulltext | true | es |
| cic.isPeerReviewed | true | es |
| cic.lugarDesarrollo | Instituto de Investigaciones Biológicas | es |
| cic.version | info:eu-repo/semantics/submittedVersion | es |
| dc.date.accessioned | 2017-04-21T14:22:54Z | |
| dc.date.available | 2017-04-21T14:22:54Z | |
| dc.identifier.uri | https://digital.cic.gba.gob.ar/handle/11746/5606 | |
| dc.title | Calcium-dependent Protein Kinases are Involved in Potato Signal Transduction. | en |
| dc.type | Artículo | es |
| dcterms.abstract | Plant response to pathogens involves an intricate network of signal transduction pathways. Here, potato cell cultures were used to study signal transduction in response to elicitors from<em>Phytophthora infestans</em>. Pretreatment of cells with Ser/Thr protein kinase inhibitors, EGTA, calmodulin antagonists or a channel blocker abolished the induction of two enzymes involved in defence responses, phenylalanine ammonia-lyase (PAL) and peroxidase. Phosphatase inhibitors caused an increase of these activities in the absence of elicitors. Hyphal cell wall components (HWC) from an incompatible race (HWC 0) produced a rapid and transient increment of histone phosphorylation, whereas induction by HWC from a compatible race (HWC C) was less pronounced and more sustained. As activities were calcium-dependent, a fraction enriched in calcium-dependent protein kinases (CDPKs) was obtained by DEAE chromatography. Fractions from HWC 0- and HWC C-treated cells presented higher kinase activity than that from untreated cells. Moreover, total activity was higher in the incompatible than in the compatible interaction. Activity was calcium-dependent, partially inhibited by calmodulin antagonists and able to phosphorylate syntide-2, a specific substrate of CDPKs. An in-gel kinase assay showed the presence of a band of approximately 50kDa whose activity was higher in HWC 0- than in HWC C-treated cells and was not detected in control extracts. This report presents evidences of the differential activation of CDPKs in response to elicitors from different races of<em>P. infestans</em>, revealing that these protein kinases participate in the defence response to oomycete. | en |
| dcterms.creator.author | Blanco, F.A. | es |
| dcterms.creator.author | Zanetti, M.E. | es |
| dcterms.creator.author | Daleo, Gustavo Raúl | es |
| dcterms.extent | 9 p. | es |
| dcterms.identifier.other | 10.1111/j.1439-0434.2007.01344.x | es |
| dcterms.identifier.url | Recurso Completo | es |
| dcterms.isPartOf.issue | vol. 156, no. 1 | es |
| dcterms.isPartOf.series | Journal of Phytopathology | es |
| dcterms.issued | 2008-01-30 | |
| dcterms.language | Inglés | es |
| dcterms.license | Attribution-NonCommercial-NoDerivatives 4.0 International (BY-NC-ND 4.0) | es |
| dcterms.subject | Calmodulin | en |
| dcterms.subject | Cell culture | en |
| dcterms.subject | Peroxidase | en |
| dcterms.subject.materia | Bioquímica y Biología Molecular | es |
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