Agrobacterium rhizogenes vs auxinic induction for in vitro rhizogenesis of Prosopis chilensis and Nothofagus alpina
| cic.isFulltext | true | es |
| cic.isPeerReviewed | true | es |
| cic.lugarDesarrollo | Universidad Nacional del Sur | es |
| cic.version | info:eu-repo/semantics/publishedVersion | es |
| dc.date.accessioned | 2016-10-04T12:24:27Z | |
| dc.date.available | 2016-10-04T12:24:27Z | |
| dc.identifier.uri | https://digital.cic.gba.gob.ar/handle/11746/4353 | |
| dc.title | Agrobacterium rhizogenes vs auxinic induction for in vitro rhizogenesis of Prosopis chilensis and Nothofagus alpina | en |
| dc.type | Artículo | es |
| dcterms.abstract | The induction and improvement of in vitro rhizogenesis of microshoots of Prosopis chilensis (Mol.) Stuntz and Nothofagus alpina (Poep. et Endl. Oerst.) were compared using Agrobacterium rhizogenes (Ar) versus indole-3-butyric acid (IBA) in the culture media. Microshoots of P. chilensis (1-2 cm length), coming from in vitro grown seedlings, were cultivated in a modified Broadleaved Tree Medium (BTMm) containing half salt concentration of macronutrients and 0.05 mg.L-1 benzilaminopurine (BAP). After 30 days, microshoots with 2-4 leaves were selected and cultured in BTMm-agar in presence or abscense of Ar and in combination with IBA. For N. alpina, the apical shoots with the first 2 true leaves, from 5 weeks old seedlings, were cultured in the abovementioned medium, but with 0.15 mgL-1 of BAP. After 2 months, microshoots with 2-3 leaves were selected and cultured in BTMm-agar, supplemented with 5 mg.L-1 IBA or in liquid BTMm on perlite and, in the presence or absence of A. rhizogenes (Ar) and in combination with 3 mg.L-1 IBA. Rooting in P. chilensis reached 100.0% when Ar infection was produced in the presence of IBA, increasing both, the number and dry weight of roots. In N. alpina, 90.0% of rooting efficiency was obtained when Ar infection was produced in liquid culture and in the absence of auxin. | en |
| dcterms.creator.author | Caro, Luis A. | es |
| dcterms.creator.author | Santecchia, Natalia | es |
| dcterms.creator.author | Marinangeli, Pablo | es |
| dcterms.creator.author | Curvetto, Néstor | es |
| dcterms.creator.author | Hernández, Luis Francisco | es |
| dcterms.extent | p. 311-318 | es |
| dcterms.identifier.other | ISSN 0327 - 9545 | es |
| dcterms.identifier.url | Documento completo | es |
| dcterms.isPartOf.issue | vol. 27, nº 3 | es |
| dcterms.isPartOf.series | Biocell | es |
| dcterms.issued | 2003 | |
| dcterms.language | Inglés | es |
| dcterms.license | Attribution-NonCommercial 4.0 International (BY-NC 4.0) | es |
| dcterms.publisher | Instituto de Histología y Embriología "Dr. Mario H. Burgos" (IHEM-CONICET) | es |
| dcterms.subject | Agrobacterium | es |
| dcterms.subject | algarrobo | es |
| dcterms.subject | hairy roots | en |
| dcterms.subject | indole-3-butyric acid | en |
| dcterms.subject | Nothofagus nervosa | es |
| dcterms.subject | Prosopis | es |
| dcterms.subject | raulí | es |
| dcterms.subject.materia | Biología Celular, Microbiología | es |
Archivos
Bloque original
1 - 1 de 1
Cargando...
- Nombre:
- Hernández_Luis_v27n3a02.pdf-PDFA.pdf
- Tamaño:
- 704.43 KB
- Formato:
- Adobe Portable Document Format
- Descripción:
- Documento completo