Isolation and characterization of a Solanum tuberosum subtilisin-like protein with caspase-3 activity (StSBTc-3)

cic.isFulltexttruees
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cic.lugarDesarrolloInstituto de Investigaciones Biológicas es
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dc.date.accessioned2017-03-16T18:15:51Z
dc.date.available2017-03-16T18:15:51Z
dc.identifier.urihttps://digital.cic.gba.gob.ar/handle/11746/5361
dc.titleIsolation and characterization of a Solanum tuberosum subtilisin-like protein with caspase-3 activity (StSBTc-3)en
dc.typeArtículoes
dcterms.abstractPlant proteases with caspase-like enzymatic activity have been widely studied during the last decade. Previously, we have reported the presence and induction of caspase-3 like activity in the apoplast of potato leaves during <em>Solanum tuberosum- Phytophthora infestans</em> interaction. In this work we have purified and identified a potato extracellular protease with caspase-3 like enzymatic activity from potato leaves infected with <em>P. infestans.</em> Results obtained from the size exclusion chromatography show that the isolated protease is a monomeric enzyme with an estimated molecular weight of 70 kDa approximately. Purified protease was analyzed by MALDI-TOF MS, showing a 100% of sequence identity with the deduced amino acid sequence of a putative subtilisin-like protease from <em>S. tuberosum </em>(Solgenomics protein ID: PGSC0003DMP400018521). For this reason the isolated protease was named as StSBTc-3. This report constitutes the first evidence of isolation and identification of a plant subtilisin-like protease with caspase-3 like enzymatic activity. In order to elucidate the possible function of StSBTc-3 during plant pathogen interaction, we demonstrate that like animal caspase-3, StSBTc-3 is able to produce in vitro cytoplasm shrinkage in plant cells and to induce plant cell death. This result suggest that, StSBTc-3 could exert a caspase executer function during potato- <em>P. infestans</em> interaction, resulting in the restriction of the pathogen spread during plantepathogen interaction.es
dcterms.creator.authorFernandez, María Belénes
dcterms.creator.authorDaleo, Gustavo Raúles
dcterms.creator.authorGuevaRA, María Gabrielaes
dcterms.extent10 p.es
dcterms.isPartOf.issuevol. 86es
dcterms.isPartOf.seriesPlant Physiology and Biochemistryes
dcterms.issued2015-01-20
dcterms.languageIngléses
dcterms.licenseAttribution-NonCommercial-NoDerivatives 4.0 International (BY-NC-ND 4.0)es
dcterms.subjectSubtilisin like proteinen
dcterms.subjectSolanum tuberosumen
dcterms.subjectDEVDaseen
dcterms.subjectApoplasten
dcterms.subjectPlantepathogen interactionen
dcterms.subject.materiaBiología Celular, Microbiologíaes

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