Isolation and characterization of a Solanum tuberosum subtilisin-like protein with caspase-3 activity (StSBTc-3)
| cic.isFulltext | true | es |
| cic.isPeerReviewed | true | es |
| cic.lugarDesarrollo | Instituto de Investigaciones Biológicas | es |
| cic.version | info:eu-repo/semantics/submittedVersion | es |
| dc.date.accessioned | 2017-03-16T18:15:51Z | |
| dc.date.available | 2017-03-16T18:15:51Z | |
| dc.identifier.uri | https://digital.cic.gba.gob.ar/handle/11746/5361 | |
| dc.title | Isolation and characterization of a Solanum tuberosum subtilisin-like protein with caspase-3 activity (StSBTc-3) | en |
| dc.type | Artículo | es |
| dcterms.abstract | Plant proteases with caspase-like enzymatic activity have been widely studied during the last decade. Previously, we have reported the presence and induction of caspase-3 like activity in the apoplast of potato leaves during <em>Solanum tuberosum- Phytophthora infestans</em> interaction. In this work we have purified and identified a potato extracellular protease with caspase-3 like enzymatic activity from potato leaves infected with <em>P. infestans.</em> Results obtained from the size exclusion chromatography show that the isolated protease is a monomeric enzyme with an estimated molecular weight of 70 kDa approximately. Purified protease was analyzed by MALDI-TOF MS, showing a 100% of sequence identity with the deduced amino acid sequence of a putative subtilisin-like protease from <em>S. tuberosum </em>(Solgenomics protein ID: PGSC0003DMP400018521). For this reason the isolated protease was named as StSBTc-3. This report constitutes the first evidence of isolation and identification of a plant subtilisin-like protease with caspase-3 like enzymatic activity. In order to elucidate the possible function of StSBTc-3 during plant pathogen interaction, we demonstrate that like animal caspase-3, StSBTc-3 is able to produce in vitro cytoplasm shrinkage in plant cells and to induce plant cell death. This result suggest that, StSBTc-3 could exert a caspase executer function during potato- <em>P. infestans</em> interaction, resulting in the restriction of the pathogen spread during plantepathogen interaction. | es |
| dcterms.creator.author | Fernandez, María Belén | es |
| dcterms.creator.author | Daleo, Gustavo Raúl | es |
| dcterms.creator.author | GuevaRA, María Gabriela | es |
| dcterms.extent | 10 p. | es |
| dcterms.isPartOf.issue | vol. 86 | es |
| dcterms.isPartOf.series | Plant Physiology and Biochemistry | es |
| dcterms.issued | 2015-01-20 | |
| dcterms.language | Inglés | es |
| dcterms.license | Attribution-NonCommercial-NoDerivatives 4.0 International (BY-NC-ND 4.0) | es |
| dcterms.subject | Subtilisin like protein | en |
| dcterms.subject | Solanum tuberosum | en |
| dcterms.subject | DEVDase | en |
| dcterms.subject | Apoplast | en |
| dcterms.subject | Plantepathogen interaction | en |
| dcterms.subject.materia | Biología Celular, Microbiología | es |
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