Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae
| cic.isFulltext | true | es |
| cic.isPeerReviewed | true | es |
| cic.lugarDesarrollo | Laboratorio de Biología Funcional y Biotecnología | es |
| cic.version | info:eu-repo/semantics/submittedVersion | es |
| dc.date.accessioned | 2017-11-24T13:11:57Z | |
| dc.date.available | 2017-11-24T13:11:57Z | |
| dc.identifier.uri | https://digital.cic.gba.gob.ar/handle/11746/6437 | |
| dc.title | Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae | en |
| dc.type | Artículo | es |
| dcterms.abstract | <em>Fusarium</em>species can produce mycotoxins, which can contaminate cereal-based food producing adverse effects for human and animal health. In recent years, the importance of<em>Fusarium poae</em>has increased within the<em>Fusarium</em>head blight complex.<em>Fusarium poae</em>is known to produce trichothecenes, especially nivalenol, a potent mycotoxin able to cause a variety of toxic effects. In this study, a specific primer pair was designed based on the<em>tri7</em>gene to detect potential nivalenol-producing<em>F. poae</em>isolates. A total of 125<em>F. poae</em>, four<em>F. cerealis</em>, two<em>F. culmorum</em>, one<em>F. langsethiae</em>, one<em>F. sporotrichioides</em>and seven<em>F. graminearum</em>, plus<em>F. austroamericanum, F. meridionale, F. graminearum sensu stricto</em>and<em>F. cortaderiae</em>from the NRRL collection were analysed, and only<em>F. poae</em>isolates gave a positive result for the presence of a 296-bp partial<em>tri7</em>DNA fragment. Moreover, the primer set was tested from cereal seed samples where<em>F. poae</em>and other<em>Fusarium</em>species with a negative result for the specific reaction (<em>F. graminearum, F. oxysporum, F. chlamydosporum, F. sporotrichioides, F. equiseti</em>and<em>F. acuminatum</em>) were isolated, and the expected fragment was amplified. We developed a rapid and reliable PCR assay to detect potential nivalenol-producing<em>F. poae</em>isolates. | en |
| dcterms.creator.author | Dinolfo, María Inés | es |
| dcterms.creator.author | Barros, Germán G. | es |
| dcterms.creator.author | Stenglein, Sebastián | es |
| dcterms.extent | 6 p. | es |
| dcterms.identifier.other | https://doi.org/10.1111/j.1574-6968.2012.02581.x | es |
| dcterms.identifier.url | Recurso Completo | es |
| dcterms.isPartOf.issue | vol. 332, no. 2 | es |
| dcterms.isPartOf.series | FEMS Microbiology Letters | es |
| dcterms.issued | 2012 | |
| dcterms.language | Inglés | es |
| dcterms.license | Attribution 4.0 International (BY 4.0) | es |
| dcterms.subject.materia | Biología Celular, Microbiología | es |
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