Examinando Artículos, Informes y presentaciones en Congresos por Autor "Eguaras, Martín Javier"
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Acceso AbiertoBroad Geographic and Host Distribution of Apis mellifera Filamentous Virus in South American Native Bees(2019) Quintana, Silvina; Fernández de Landa, Gregorio; Revainera, Pablo; Meroi Arcerito, Facundo René; Porrini, Leonardo Pablo; Di Geronimo, Vanesa; Brasesco, María Cosntanza; Plischuk, Santiago; Eguaras, Martín Javier; Maggi, MatíasApis mellifera filamentous virus (AmFV) is a large double stranded DNA virus of honey bees and its prevalence and relationship with other parasites is poorly known. Samples consisted of fifty-one adult bees belonging to eight native species collected using entomological nets in six provinces of Argentina, from 2009 to 2018. Total genomic DNA was extracted from individual bees and a 551 bp fragment of the Bro-N gene of AmFV was amplified by qPCR. In the present work we have reported for the first time both the presence and the wide geographic distribution of AmFV in Argentinian species of native bees. This is the first report of the presence of this virus associated with Xylocopa atamisquensis, X. augusti, X. frontalis, X. spendidula, Bombus pauloensis and Peponapis fervens. Detecting pathogens that could threaten native bee health is of outmost importance to generate both conservation and management strategies.
Acceso AbiertoComparison of qPCR and Morphological Methods For Detection of Acarapis Woodi in Honey Bee Samples(2019) Quintana, Silvina; Szawarski, Nicolás; Sarlo, Gabriel; Medici, Sandra; Rivero, Mariana; Eguaras, Martín Javier; Maggi, MatíasThe honey bee tracheal mite Acarapis woodi is an internal obligate parasite of adult honey bees (Apis mellifera). The small size of the mites and location within the bee’s trachea create a challenge for diagnostic identification. Detailed assessment of low-level mite infestation involves microscopic examination of the bee’s tracheae, but this traditional diagnostic method takes a long time. In recent years, new molecular diagnostic techniques have been developed to identify A. woodi with the use of PCRs. The purpose of this study was to compare the diagnostic sensitivity of microscopic examination and qPCR method for the detection of A. woodi in honey bee samples. Thirty-six samples were analyzed with both methods and qPCR was more sensitive than the microscopic method to detect A. woodi. This work demonstrates the practical application of molecular technology as a support tool for surveys and contingency management and to provide robust surveillance data on the presence or absence of A. woodi in honey bee colonies.
Acceso AbiertoEffect of Abscisic Acid (ABA) Combined with Two Different Beekeeping Nutritional Strategies to Confront Overwintering(2019) Szawarski, Nicolás; Saez, Agustín; Dominguez, Enzo; Dickson, Rachel; De Matteis, Angela; Eciolaza, Carlos; Justel, Marcelino; Aliano, Alfredo; Solar, Pedro; Bergara, Ignacio; Pons, Claudia Fabiana; Bolognesi, Aldo; Carna, Gabriel; Garcia, Walter; Garcia, Omar; Eguaras, Martín Javier; Lamattina, Lorenzo; Maggi, Matías; Negri, PedroIn temperate climates, beekeeping operations suffer colony losses and colony depopulation of Apis mellifera during overwintering, which are associated with biotic and abiotic stressors that impact bees’ health. In this work, we evaluate the impacts of abscisic acid (ABA) dietary supplementation on honey bee colonies kept in Langstroth hives. The effects of ABA were evaluated in combination with two different beekeeping nutritional strategies to confront overwintering: “honey management” and “syrup management”. Specifically, we evaluated strength parameters of honey bee colonies (adult bee and brood population) and the population dynamics of Nosema (prevalence and intensity) associated with both nutritional systems and ABA supplementation during the whole study (late autumn-winter-early spring). The entire experiment was designed and performed with a local group of beekeepers, “Azahares del sudeste”, who showed interest in answering problems associated with the management of honey bee colonies during the winter. The results indicated that the ABA supplementation had positive effects on the population dynamics of the A. mellifera colonies during overwintering and on the nosemosis at colony level (prevalence) in both nutritional strategies evaluated.