Artículos, Informes y presentaciones en Congresos
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Acceso Abierto Effect of Abscisic Acid (ABA) Combined with Two Different Beekeeping Nutritional Strategies to Confront Overwintering(2019) Szawarski, Nicolás; Saez, Agustín; Dominguez, Enzo; Dickson, Rachel; De Matteis, Angela; Eciolaza, Carlos; Justel, Marcelino; Aliano, Alfredo; Solar, Pedro; Bergara, Ignacio; Pons, Claudia Fabiana; Bolognesi, Aldo; Carna, Gabriel; Garcia, Walter; Garcia, Omar; Eguaras, Martín Javier; Lamattina, Lorenzo; Maggi, Matías; Negri, PedroIn temperate climates, beekeeping operations suffer colony losses and colony depopulation of Apis mellifera during overwintering, which are associated with biotic and abiotic stressors that impact bees’ health. In this work, we evaluate the impacts of abscisic acid (ABA) dietary supplementation on honey bee colonies kept in Langstroth hives. The effects of ABA were evaluated in combination with two different beekeeping nutritional strategies to confront overwintering: “honey management” and “syrup management”. Specifically, we evaluated strength parameters of honey bee colonies (adult bee and brood population) and the population dynamics of Nosema (prevalence and intensity) associated with both nutritional systems and ABA supplementation during the whole study (late autumn-winter-early spring). The entire experiment was designed and performed with a local group of beekeepers, “Azahares del sudeste”, who showed interest in answering problems associated with the management of honey bee colonies during the winter. The results indicated that the ABA supplementation had positive effects on the population dynamics of the A. mellifera colonies during overwintering and on the nosemosis at colony level (prevalence) in both nutritional strategies evaluated. - Artículo
Acceso Abierto Natural molecules for the control of Paenibacillus larvae, causal agent of American foulbrood in honey bees (Apis mellifera L.)(2019) Giménez Martínez, Pablo Darian; Cugnata, Noelia; Alonso Salces, Rosa María; Arredondo, Daniela; Antúnez, Karina; De Castro, Rosana Esther; Fuselli, Sandra RosaAim of study: To evaluate the potential bactericidal activity of natural molecules against Paenibacillus larvae. Moreover, we investigated if molecules that exhibit antimicrobial activity were able to inhibit the proteolytic activity of the bacterium. Area of study: Isolates S1 and S2 were from Balcarce, Buenos Aires province, strain S3 from Rio Cuarto, Cordoba province, strain S4 from Concordia, Entre Rios province, strain S5 and S8 from Necochea, Buenos Aires, strain S6 and S7 from Mar del Plata, Buenos Aires, strain S9 from Modena, Italy and strain S10 from Emilia Reggio, Italy. Material and methods: Bacterial isolates identification was carried out by amplification of a specific 16S rRNA gene fragment of P. larvae using primers PL5 and PL4. Screening of the antimicrobial activity of thirteen molecules against four P. larvae isolates was conducted by the agar diffusion technique. The antimicrobial activity of selected molecules was evaluated by broth microdilution method. Main results: Menadione, lauric acid, monoglyceride of lauric acid and naringenin showed antimicrobial activity against ten P. larvae isolates. Menadione and lauric acid showed the strongest activities, with minimum inhibitory concentration mean values ranging 0.78-3.125 μg/mL and 25-50 μg/mL, respectively. Research highlights: Those concentrations are feasible to be applied at field level, and constitute promissory candidates to be evaluated using in vivo larval models. - Comunicacion
Acceso Abierto Broad Geographic and Host Distribution of Apis mellifera Filamentous Virus in South American Native Bees(2019) Quintana, Silvina; Fernández de Landa, Gregorio; Revainera, Pablo; Meroi Arcerito, Facundo René; Porrini, Leonardo Pablo; Di Geronimo, Vanesa; Brasesco, María Cosntanza; Plischuk, Santiago; Eguaras, Martín Javier; Maggi, MatíasApis mellifera filamentous virus (AmFV) is a large double stranded DNA virus of honey bees and its prevalence and relationship with other parasites is poorly known. Samples consisted of fifty-one adult bees belonging to eight native species collected using entomological nets in six provinces of Argentina, from 2009 to 2018. Total genomic DNA was extracted from individual bees and a 551 bp fragment of the Bro-N gene of AmFV was amplified by qPCR. In the present work we have reported for the first time both the presence and the wide geographic distribution of AmFV in Argentinian species of native bees. This is the first report of the presence of this virus associated with Xylocopa atamisquensis, X. augusti, X. frontalis, X. spendidula, Bombus pauloensis and Peponapis fervens. Detecting pathogens that could threaten native bee health is of outmost importance to generate both conservation and management strategies. - Artículo
Acceso Abierto Comparison of qPCR and Morphological Methods For Detection of Acarapis Woodi in Honey Bee Samples(2019) Quintana, Silvina; Szawarski, Nicolás; Sarlo, Gabriel; Medici, Sandra; Rivero, Mariana; Eguaras, Martín Javier; Maggi, MatíasThe honey bee tracheal mite Acarapis woodi is an internal obligate parasite of adult honey bees (Apis mellifera). The small size of the mites and location within the bee’s trachea create a challenge for diagnostic identification. Detailed assessment of low-level mite infestation involves microscopic examination of the bee’s tracheae, but this traditional diagnostic method takes a long time. In recent years, new molecular diagnostic techniques have been developed to identify A. woodi with the use of PCRs. The purpose of this study was to compare the diagnostic sensitivity of microscopic examination and qPCR method for the detection of A. woodi in honey bee samples. Thirty-six samples were analyzed with both methods and qPCR was more sensitive than the microscopic method to detect A. woodi. This work demonstrates the practical application of molecular technology as a support tool for surveys and contingency management and to provide robust surveillance data on the presence or absence of A. woodi in honey bee colonies. - Documento de conferencia
Acceso Abierto Presentación y Líneas de Investigación Institucionales(2018) Denegri., Guillermo M.El IIPROSAM es un instituto de investigación que realiza actividades científicas y técnicas, de docencia de grado y posgrado, de formación de recursos humanos, de extensión y de servicios de transferencia de tecnología a terceras instituciones públicas o privadas. Con estos objetivos compartidos o relacionados que los vinculan, se reunieron en una misma estructura, profesionales que tienen una importante historia de interacciones científico-académicas y de vinculación entre sí. Los proyectos de investigación y desa- rrollo en curso, enfocan problemáticas vinculadas al manejo integrado de plagas, estudio de las parasito- sis de importancia sanitaria, con especial énfasis en formas de control no contaminantes, la diversidad y el cuidado del medio ambiente. El IIPROSAM cuenta con aproximadamente 70 personas en su plantel, donde la pirámide está equilibrada en lo que respecta a la cantidad de investigadores en sus distintas categorías, becarios posdoctorales y becarios doctorales. La interacción fecunda entre la Universidad Nacional de Mar del Plata, el Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) y la Comisión de Inves- tigaciones Científicas de la provincia de Buenos Aires (CIC), tiene como objetivo fundamental aportar a la generación de conocimiento básico, aplicado y desarrollo tecnológico, que permita abordar problemáticas regionales, provinciales y nacionales que mejoren la calidad de vida de nuestros conciudadanos que son en definitiva la razón de ser de nuestra actividad.