Show simple item record 2016-10-19T12:13:25Z 2016-10-19T12:13:25Z
dc.title Novel Vanadium-Loaded Ordered Collagen Scaffold Promotes Osteochondral Differentiation of Bone Marrow Progenitor Cells en
dc.type Artículo es
dcterms.abstract Bone and cartilage regeneration can be improved by designing a functionalized biomaterial that includes bioactive drugs in a biocompatible and biodegradable scaffold. Based on our previous studies, we designed a vanadium-loaded collagen scaffold for osteochondral tissue engineering. Collagen-vanadium loaded scaffolds were characterized by SEM, FTIR, and permeability studies. Rat bone marrow progenitor cells were plated on collagen or vanadium-loaded membranes to evaluate differences in cell attachment, growth and osteogenic or chondrocytic differentiation. The potential cytotoxicity of the scaffolds was assessed by the MTT assay and by evaluation of morphological changes in cultured RAW 264.7 macrophages. Our results show that loading of VOAsc did not alter the grooved ordered structure of the collagen membrane although it increased membrane permeability, suggesting a more open structure. The VOAsc was released to the media, suggesting diffusion-controlled drug release. Vanadium-loaded membranes proved to be a better substratum than for all evaluated aspects of BMPC biocompatibility (adhesion, growth, and osteoblastic and chondrocytic differentiation). In addition, there was no detectable effect of collagen or vanadium-loaded scaffolds on macrophage viability or cytotoxicity. Based on these findings, we have developed a new ordered collagen scaffold loaded with VOAsc that shows potential for osteochondral tissue engineering. en
dcterms.extent 12 p. es
dcterms.issued 2016
dcterms.language Inglés es
dcterms.license Attribution 4.0 International (BY 4.0) es
dcterms.subject bone regeneration en
dcterms.subject cartilage regeneration en
dcterms.subject bioactive drugs en
dcterms.subject biocompatible and biodegradable scaffold en
dcterms.subject osteochondral tissue engineering en
cic.version info:eu-repo/semantics/publishedVersion es Cortizo, Ana María es Ruderman, Graciela es Mazzini, Flavia N. es Molinuevo, M. Silvina es Mogilner, Ines G. es
cic.lugarDesarrollo Universidad Nacional de La Plata es
dcterms.subject.materia Ciencias Químicas es
dcterms.identifier.url Recurso Completo es
dcterms.isPartOf.issue vol. 2016 es
dcterms.isPartOf.series International Journal of Biomaterials es
cic.isPeerReviewed true es
cic.isFulltext true es


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