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dc.title Comparison of low molecular weight glutenin subunits identified by SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR in common wheat es
dc.type Artículo es
dcterms.abstract <strong>Background</strong> Low-molecular-weight glutenin subunits (LMW-GS) play a crucial role in determining end-use quality of common wheat by influencing the viscoelastic properties of dough. Four different methods - sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), two-dimensional gel electrophoresis (2-DE, IEF × SDS-PAGE), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and polymerase chain reaction (PCR), were used to characterize the LMW-GS composition in 103 cultivars from 12 countries. <strong>Results</strong> At the<em>Glu-A3</em>locus, all seven alleles could be reliably identified by 2-DE and PCR. However, the alleles<em>Glu-A3e</em>and<em>Glu-A3d</em>could not be routinely distinguished from<em>Glu-A3f</em>and<em>Glu-A3g</em>, respectively, based on SDS-PAGE, and the allele<em>Glu-A3a</em>could not be differentiated from<em>Glu-A3c</em>by MALDI-TOF-MS. At the<em>Glu-B3</em>locus, alleles<em>Glu-B3a</em>,<em>Glu-B3b</em>,<em>Glu-B3c</em>,<em>Glu-B3g</em>,<em>Glu-B3h</em>and<em>Glu-B3j</em>could be clearly identified by all four methods, whereas<em>Glu-B3ab</em>,<em>Glu-B3ac</em>,<em>Glu-B3ad</em>could only be identified by the 2-DE method. At the<em>Glu-D3</em>locus, allelic identification was problematic for the electrophoresis based methods and PCR. MALDI-TOF-MS has the potential to reliably identify the<em>Glu-D3</em>alleles. <strong>Conclusions</strong> PCR is the simplest, most accurate, lowest cost, and therefore recommended method for identification of<em>Glu-A3</em>and<em>Glu-B3</em>alleles in breeding programs. A combination of methods was required to identify certain alleles, and would be especially useful when characterizing new alleles. A standard set of 30 cultivars for use in future studies was chosen to represent all LMW-GS allelic variants in the collection. Among them, Chinese Spring, Opata 85, Seri 82 and Pavon 76 were recommended as a core set for use in SDS-PAGE gels.<em>Glu-D3c</em>and<em>Glu-D3e</em>are the same allele. Two new alleles, namely,<em>Glu-D3m</em>in cultivar Darius, and<em>Glu-D3n</em>in Fengmai 27, were identified by 2-DE. Utilization of the suggested standard cultivar set, seed of which is available from the CIMMYT and INRA Clermont-Ferrand germplasm collections, should also promote information sharing in the identification of individual LMW-GS and thus provide useful information for quality improvement in common wheat. en
dcterms.extent 24 p. es
dcterms.issued 2010
dcterms.language Inglés es
dcterms.license Attribution 4.0 International (BY 4.0) es
dcterms.subject Common Wheat en
dcterms.subject Glutenin Subunit en
dcterms.subject Standard Cultivar en
dcterms.subject Glutenin Protein en
dcterms.subject Glutenin Extract en
cic.version info:eu-repo/semantics/submittedVersion es Liu, Li es Ikeda, Tatsuya M. es Branlard, Gerard es Peña, Roberto J. es Rogers, John William es Lerner, Silvia E. es Kolman, Maía de Los Angeles es Xia, Xianchun es Wang, Linhai es Ma, Wujun es Appels, Rudi es Yoshida, Hisashi es Wang, Aili es Yan, Yueming es He, Zhonghu es
cic.lugarDesarrollo Laboratorio de Biología Funcional y Biotecnología es
dcterms.subject.materia Agronomía, reproducción y protección de plantas es
dcterms.identifier.url Recurso Completo es
dcterms.identifier.other doi:10.1186/1471-2229-10-124 es
dcterms.isPartOf.issue vol. 210 es
dcterms.isPartOf.series BMC Plant Biology es
cic.isPeerReviewed true es
cic.isFulltext true es


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