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dc.date.accessioned 2018-06-21T16:46:28Z
dc.date.available 2018-06-21T16:46:28Z
dc.identifier.uri http://digital.cic.gba.gob.ar/handle/11746/8275
dc.title Trypanosoma cruzi serinecarboxipeptidaseis a sufated glycoprotein and a minor antigen in human Chagas disease infection en
dc.type Artículo es
dcterms.abstract In this work, the presence of sulfated N-glycans was studied in a high-mannose-type glycoprotein of <em>Trypanosoma cruzi</em> with serinecarboxipeptidase (T<em>c</em>SCP) activity. The immune cross-reactivity between purified SCP and Cruzipain (Cz) was evidenced using rabbit sera specific for both glycoproteins. Taking advantage that SCP co-purifies with Cz from Concanavalin-A affinity columns, the Cz–SCP mixture was desulfated, ascribing the cross-reactivity to the presence of sulfate groups in both molecules. Therefore, knowing that Cz is a sulfated glycoprotein, with antigenic sulfated epitopes (sulfotopes), SCP was excised from SDS-PAGE and the N-glycosydic chains were analyzed by UV–MALDI–TOF-MS, confirming the presence of short-sulfated high-mannose-type oligosaccharidic chains. Besides, the presence of sulfotopes was analyzed in lysates of the different parasite stages demonstrating that a band with apparent molecular weight similar to SCP was highly recognized in trypomastigotes. In addition, SCP was confronted with sera of infected people with different degrees of cardiac dysfunction. Although most sera recognized it in different groups, no statistical association was found between sera antibodies specific for SCP and the severity of the disease. In summary, our findings demonstrate (1) the presence of sulfate groups in the N-glycosidic short chains of native T<em>c</em>SCP, (2) the existence of immune cross-reactivity between Cz and SCP, purified from epimastigotes, (3) the presence of common sulfotopes between both parasite glycoproteins, and (4) the enhanced presence of sulfotopes in trypomastigotes, probably involved in parasite–host relationship and/or infection. Interestingly, we show for the first time that SCP is a minor antigen recognized by most of chronic Chagas disease patient’s sera. en
dcterms.extent 12 p. es
dcterms.issued 2017-12-22
dcterms.language Inglés es
dcterms.license Attribution-NonCommercial-NoDerivatives 4.0 International (BY-NC-ND 4.0) es
dcterms.subject Trypanosoma cruzi es
dcterms.subject serinecarboxipeptidase en
dcterms.subject immune cross-reactivity en
dcterms.subject glycoprotein en
cic.version info:eu-repo/semantics/submittedVersion es
dcterms.creator.author Soprano, Luciana L. es
dcterms.creator.author Parente, Juliana Elena es
dcterms.creator.author Landoni, Malena es
dcterms.creator.author Couto, Alicia S. es
dcterms.creator.author Duschak, Vilma G. es
cic.lugarDesarrollo Planta Piloto Multipropósito - Laboratorio de Servicios a la Industria y al Sistema Científico es
dcterms.subject.materia Ciencias Médicas y de la Salud es
dcterms.identifier.other doi:10.1007/s00430-017-0529-7 es
dcterms.isPartOf.issue vol. 207, no. 2 es
dcterms.isPartOf.series Medical Microbiology and Immunology es
cic.isPeerReviewed true es
cic.isFulltext true es


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