Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects against Bordetella pertussis Infection
| cic.isFulltext | true | es |
| cic.isPeerReviewed | true | es |
| cic.lugarDesarrollo | Universidad Nacional de La Plata | es |
| cic.version | info:eu-repo/semantics/publishedVersion | es |
| dc.date.accessioned | 2018-03-27T12:42:06Z | |
| dc.date.available | 2018-03-27T12:42:06Z | |
| dc.identifier.uri | https://digital.cic.gba.gob.ar/handle/11746/7069 | |
| dc.title | Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects against Bordetella pertussis Infection | en |
| dc.type | Artículo | es |
| dcterms.abstract | This study examined the immunogenic properties of the fusion protein fimbria 2 of Bordetella pertussis (Fim2)—cholera toxin B subunit (CTB) in the intranasal murine model of infection. To this end B. pertussis Fim2 coding sequence was cloned downstream of the cholera toxin B subunit coding sequence.Theexpression and assembly of the fusion protein into pentameric structures (CTBFim2) were evaluated by SDS-PAGE and monosialotetrahexosylgaglioside (GM1-ganglioside) enzyme-linked immunosorbent assay (ELISA). To evaluate the protective capacity of CTB-Fim2, an intraperitoneal or intranasal mouse immunization schedule was performed with 50 μg of CTB-Fim2. Recombinant (rFim2) or purified (BpFim2) Fim2, CTB, and phosphate-buffered saline (PBS) were used as controls. The results showed that mice immunized with BpFim2 or CTB-Fim2 intraperitoneally or intranasally presented a significant reduction in bacterial lung counts compared to control groups (P < 0.01 or P < 0.001, resp.). Moreover, intranasal immunization with CTB-Fim2 induced significant levels of Fim2-specific IgG in serum and bronchoalveolar lavage (BAL) and Fim2-specific IgA in BAL. Analysis of IgG isotypes and cytokines mRNA levels showed that CTB-Fim2 results in a mixed Th1/Th2 (T-helper) response. The data presented here provide support for CTB-Fim2 as a promising recombinant antigen against Bordetella pertussis infection. | en |
| dcterms.creator.author | Olivera, Noelia | es |
| dcterms.creator.author | Castuma, Celina E. | es |
| dcterms.creator.author | Hozbor, Daniela Flavia | es |
| dcterms.creator.author | Gaillard, María E. | es |
| dcterms.creator.author | Rumbo, Martín | es |
| dcterms.creator.author | Gómez, Ricardo M. | es |
| dcterms.extent | 12 p. | es |
| dcterms.identifier.other | DOI: 10.1155/2014/421486 | es |
| dcterms.identifier.url | Recurso online | es |
| dcterms.isPartOf.issue | vol. 2014 | es |
| dcterms.isPartOf.item | Informe científico de investigador: Hozbor, Daniela Flavia (2012-2013) | es |
| dcterms.isPartOf.series | BioMed Research International | es |
| dcterms.issued | 2014 | |
| dcterms.language | Inglés | es |
| dcterms.license | Attribution 4.0 International (BY 4.0) | es |
| dcterms.publisher | Hindawi Publishing Corporation | es |
| dcterms.subject | Bordetella pertussis | es |
| dcterms.subject | cholera toxin B | en |
| dcterms.subject.materia | Bioquímica y Biología Molecular | es |
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