Proteasas de Bromeliaceae: V. Separación y purificación de sulfhidril - proteasas presentes en frutos de Bromelia balaasae Mez
| cic.isFulltext | true | es |
| cic.isPeerReviewed | true | es |
| cic.lugarDesarrollo | Universidad Nacional de La Plata | es |
| cic.parentType | Articulo | |
| cic.version | info:eu-repo/semantics/submittedVersion | es |
| dc.date.accessioned | 2016-08-16T18:09:05Z | |
| dc.date.available | 2016-08-16T18:09:05Z | |
| dc.identifier.uri | https://digital.cic.gba.gob.ar/handle/11746/3694 | |
| dc.title | Proteasas de Bromeliaceae: V. Separación y purificación de sulfhidril - proteasas presentes en frutos de Bromelia balaasae Mez | es |
| dc.type | Artículo | es |
| dcterms.abstract | Almost mature fruits of Bromelia serra Griseb., blade homogenized (Waring blender) with cold acetone (-20ºC), provide a light, proteolitically active acetone powder (1, 93g%, w/w). Extractives obtained by gentle stirring with phosphate buffer pH 6,4 containing 5 mM cysteine and 5 mM EDTA (protein content 8, 24 g per 100 g of powder) show good thermal stability at moderates temperatures and highest caseinolytic activity at pH 6.0-7.8. Gel- filtration (Sephadex G-75, Superfine) allows to separate one active fraction which is resolved in two new, more pursed fractions through ion-exchange chromatography (DEAE Sephacel and CM Sepharose). | en |
| dcterms.creator.author | Caffini, Néstor Oscar | es |
| dcterms.creator.author | Priolo de Lufrano, Nora Silvia | es |
| dcterms.creator.author | Buttazzoni de Cozzarin, Marta Susana | es |
| dcterms.creator.author | Natalucci, Claudia Luisa | es |
| dcterms.extent | 6 p. | es |
| dcterms.identifier.url | Registro completo | es |
| dcterms.isPartOf.issue | vol. 7, no. 1 | es |
| dcterms.isPartOf.series | Acta Farmacéutica Bonaerense | es |
| dcterms.issued | 1988-01-01 | |
| dcterms.language | Español | es |
| dcterms.license | Attribution 4.0 International (BY 4.0) | es |
| dcterms.subject | Hidrolasas de péptidos | es |
| dcterms.subject | Enzimas | es |
| dcterms.subject | Cromatografía | es |
| dcterms.subject.materia | Farmacología y Farmacia | es |
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