Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina.

cic.isFulltexttruees
cic.isPeerReviewedtruees
cic.lugarDesarrolloCentro de Investigaciones de Fitopatología es
cic.versioninfo:eu-repo/semantics/submittedVersiones
dc.date.accessioned2018-04-23T12:37:24Z
dc.date.available2018-04-23T12:37:24Z
dc.identifier.urihttps://digital.cic.gba.gob.ar/handle/11746/7195
dc.titleDiversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina.en
dc.typeArtículoes
dcterms.abstractThe aim of this study was to isolate, identify and analyze the diversity of the causative agents of crown galls and hairy roots from symptomatic plants of<em>Vaccinium corymbossum</em>by means of biological, biochemical and molecular tools. All the bacteria isolated from blueberries (<em>n</em> = 78) were found to be<em>Agrobacterium</em>since they grew on three differential media, provoked cell and/or root proliferation on Kalanchoe, and contained a 730bp partial sequence that codes for virulence genes within the<em>vir</em>C operon found on Ti and/or Ri plasmids. Isolates were highly variable considering the ERIC-PCR patterns as well as biochemical reactions and were all represented by 7 different restriction patterns of the 16SrDNA. While most of the isolates belonged to<em>Agrobacterium</em>bv. 1 (<em>n</em> = 33) or<em>Agrobacterium</em>bv. 2 (<em>n</em> = 31) only fourteen were<em>Agrobacterium rubi.</em>A representative isolate of each of these three groups was further identified by sequencing the approximately 400bp 16SrDNA. We concluded that<em>Vaccinium</em>plants are particularly susceptible to<em>Agrobacterium</em>bv. 1,<em>Agrobacterium</em>bv. 2, and also to<em>Agrobacterium rubi.</em>To our knowledge this is the first survey of<em>Agrobacterium</em>affecting blueberries in Argentina.en
dcterms.creator.authorAlippi, Adriana Mónicaes
dcterms.creator.authorLopez Ana Claudiaes
dcterms.creator.authorBalatti, Pedro Albertoes
dcterms.extent16 p.es
dcterms.identifier.urlRecurso Completoes
dcterms.isPartOf.issuevol. 134, no. 2es
dcterms.isPartOf.seriesEuropean Journal of Plant Pathologyes
dcterms.issued2012-10-10
dcterms.languageIngléses
dcterms.licenseAttribution-NonCommercial-NoDerivatives 4.0 International (BY-NC-ND 4.0)es
dcterms.subjectAgrobacteriumen
dcterms.subjectCROWN GALLen
dcterms.subjectAgrobacterium rubien
dcterms.subjectAgrobacterium tumefaciensen
dcterms.subjectAgrobacterium rhizogenesen
dcterms.subjectBiovarsen
dcterms.subjectVaccinium corymbossumen
dcterms.subjectBlueberriesen
dcterms.subjectCrown gall diseaseen
dcterms.subjectDiversityen
dcterms.subjectPCRen
dcterms.subject.materiaAgronomía, reproducción y protección de plantases

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