Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina.

cic.isFulltext true es
cic.isPeerReviewed true es
cic.lugarDesarrollo Centro de Investigaciones de Fitopatología es
cic.version info:eu-repo/semantics/submittedVersion es
dc.date.accessioned 2018-04-23T12:37:24Z
dc.date.available 2018-04-23T12:37:24Z
dc.identifier.uri https://digital.cic.gba.gob.ar/handle/11746/7195
dc.title Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina. en
dc.type Artículo es
dcterms.abstract The aim of this study was to isolate, identify and analyze the diversity of the causative agents of crown galls and hairy roots from symptomatic plants of<em>Vaccinium corymbossum</em>by means of biological, biochemical and molecular tools. All the bacteria isolated from blueberries (<em>n</em> = 78) were found to be<em>Agrobacterium</em>since they grew on three differential media, provoked cell and/or root proliferation on Kalanchoe, and contained a 730bp partial sequence that codes for virulence genes within the<em>vir</em>C operon found on Ti and/or Ri plasmids. Isolates were highly variable considering the ERIC-PCR patterns as well as biochemical reactions and were all represented by 7 different restriction patterns of the 16SrDNA. While most of the isolates belonged to<em>Agrobacterium</em>bv. 1 (<em>n</em> = 33) or<em>Agrobacterium</em>bv. 2 (<em>n</em> = 31) only fourteen were<em>Agrobacterium rubi.</em>A representative isolate of each of these three groups was further identified by sequencing the approximately 400bp 16SrDNA. We concluded that<em>Vaccinium</em>plants are particularly susceptible to<em>Agrobacterium</em>bv. 1,<em>Agrobacterium</em>bv. 2, and also to<em>Agrobacterium rubi.</em>To our knowledge this is the first survey of<em>Agrobacterium</em>affecting blueberries in Argentina. en
dcterms.creator.author Alippi, Adriana Mónica es
dcterms.creator.author Lopez Ana Claudia es
dcterms.creator.author Balatti, Pedro Alberto es
dcterms.extent 16 p. es
dcterms.identifier.url Recurso Completo es
dcterms.isPartOf.issue vol. 134, no. 2 es
dcterms.isPartOf.series European Journal of Plant Pathology es
dcterms.issued 2012-10-10
dcterms.language Inglés es
dcterms.license Attribution-NonCommercial-NoDerivatives 4.0 International (BY-NC-ND 4.0) es
dcterms.subject Agrobacterium en
dcterms.subject CROWN GALL en
dcterms.subject Agrobacterium rubi en
dcterms.subject Agrobacterium tumefaciens en
dcterms.subject Agrobacterium rhizogenes en
dcterms.subject Biovars en
dcterms.subject Vaccinium corymbossum en
dcterms.subject Blueberries en
dcterms.subject Crown gall disease en
dcterms.subject Diversity en
dcterms.subject PCR en
dcterms.subject.materia Agronomía, reproducción y protección de plantas es
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