Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina.
Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina.
| cic.isFulltext | true | es |
| cic.isPeerReviewed | true | es |
| cic.lugarDesarrollo | Centro de Investigaciones de Fitopatología | es |
| cic.version | info:eu-repo/semantics/submittedVersion | es |
| dc.date.accessioned | 2018-04-23T12:37:24Z | |
| dc.date.available | 2018-04-23T12:37:24Z | |
| dc.identifier.uri | https://digital.cic.gba.gob.ar/handle/11746/7195 | |
| dc.title | Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina. | en |
| dc.type | Artículo | es |
| dcterms.abstract | The aim of this study was to isolate, identify and analyze the diversity of the causative agents of crown galls and hairy roots from symptomatic plants of<em>Vaccinium corymbossum</em>by means of biological, biochemical and molecular tools. All the bacteria isolated from blueberries (<em>n</em> = 78) were found to be<em>Agrobacterium</em>since they grew on three differential media, provoked cell and/or root proliferation on Kalanchoe, and contained a 730bp partial sequence that codes for virulence genes within the<em>vir</em>C operon found on Ti and/or Ri plasmids. Isolates were highly variable considering the ERIC-PCR patterns as well as biochemical reactions and were all represented by 7 different restriction patterns of the 16SrDNA. While most of the isolates belonged to<em>Agrobacterium</em>bv. 1 (<em>n</em> = 33) or<em>Agrobacterium</em>bv. 2 (<em>n</em> = 31) only fourteen were<em>Agrobacterium rubi.</em>A representative isolate of each of these three groups was further identified by sequencing the approximately 400bp 16SrDNA. We concluded that<em>Vaccinium</em>plants are particularly susceptible to<em>Agrobacterium</em>bv. 1,<em>Agrobacterium</em>bv. 2, and also to<em>Agrobacterium rubi.</em>To our knowledge this is the first survey of<em>Agrobacterium</em>affecting blueberries in Argentina. | en |
| dcterms.creator.author | Alippi, Adriana Mónica | es |
| dcterms.creator.author | Lopez Ana Claudia | es |
| dcterms.creator.author | Balatti, Pedro Alberto | es |
| dcterms.extent | 16 p. | es |
| dcterms.identifier.url | Recurso Completo | es |
| dcterms.isPartOf.issue | vol. 134, no. 2 | es |
| dcterms.isPartOf.series | European Journal of Plant Pathology | es |
| dcterms.issued | 2012-10-10 | |
| dcterms.language | Inglés | es |
| dcterms.license | Attribution-NonCommercial-NoDerivatives 4.0 International (BY-NC-ND 4.0) | es |
| dcterms.subject | Agrobacterium | en |
| dcterms.subject | CROWN GALL | en |
| dcterms.subject | Agrobacterium rubi | en |
| dcterms.subject | Agrobacterium tumefaciens | en |
| dcterms.subject | Agrobacterium rhizogenes | en |
| dcterms.subject | Biovars | en |
| dcterms.subject | Vaccinium corymbossum | en |
| dcterms.subject | Blueberries | en |
| dcterms.subject | Crown gall disease | en |
| dcterms.subject | Diversity | en |
| dcterms.subject | PCR | en |
| dcterms.subject.materia | Agronomía, reproducción y protección de plantas | es |
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