Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina.
cic.isFulltext | true | es |
cic.isPeerReviewed | true | es |
cic.lugarDesarrollo | Centro de Investigaciones de Fitopatología | es |
cic.version | info:eu-repo/semantics/submittedVersion | es |
dc.date.accessioned | 2018-04-23T12:37:24Z | |
dc.date.available | 2018-04-23T12:37:24Z | |
dc.identifier.uri | https://digital.cic.gba.gob.ar/handle/11746/7195 | |
dc.title | Diversity among strains of four Agrobacterium species isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina. | en |
dc.type | Artículo | es |
dcterms.abstract | The aim of this study was to isolate, identify and analyze the diversity of the causative agents of crown galls and hairy roots from symptomatic plants of<em>Vaccinium corymbossum</em>by means of biological, biochemical and molecular tools. All the bacteria isolated from blueberries (<em>n</em> = 78) were found to be<em>Agrobacterium</em>since they grew on three differential media, provoked cell and/or root proliferation on Kalanchoe, and contained a 730bp partial sequence that codes for virulence genes within the<em>vir</em>C operon found on Ti and/or Ri plasmids. Isolates were highly variable considering the ERIC-PCR patterns as well as biochemical reactions and were all represented by 7 different restriction patterns of the 16SrDNA. While most of the isolates belonged to<em>Agrobacterium</em>bv. 1 (<em>n</em> = 33) or<em>Agrobacterium</em>bv. 2 (<em>n</em> = 31) only fourteen were<em>Agrobacterium rubi.</em>A representative isolate of each of these three groups was further identified by sequencing the approximately 400bp 16SrDNA. We concluded that<em>Vaccinium</em>plants are particularly susceptible to<em>Agrobacterium</em>bv. 1,<em>Agrobacterium</em>bv. 2, and also to<em>Agrobacterium rubi.</em>To our knowledge this is the first survey of<em>Agrobacterium</em>affecting blueberries in Argentina. | en |
dcterms.creator.author | Alippi, Adriana Mónica | es |
dcterms.creator.author | Lopez Ana Claudia | es |
dcterms.creator.author | Balatti, Pedro Alberto | es |
dcterms.extent | 16 p. | es |
dcterms.identifier.url | Recurso Completo | es |
dcterms.isPartOf.issue | vol. 134, no. 2 | es |
dcterms.isPartOf.series | European Journal of Plant Pathology | es |
dcterms.issued | 2012-10-10 | |
dcterms.language | Inglés | es |
dcterms.license | Attribution-NonCommercial-NoDerivatives 4.0 International (BY-NC-ND 4.0) | es |
dcterms.subject | Agrobacterium | en |
dcterms.subject | CROWN GALL | en |
dcterms.subject | Agrobacterium rubi | en |
dcterms.subject | Agrobacterium tumefaciens | en |
dcterms.subject | Agrobacterium rhizogenes | en |
dcterms.subject | Biovars | en |
dcterms.subject | Vaccinium corymbossum | en |
dcterms.subject | Blueberries | en |
dcterms.subject | Crown gall disease | en |
dcterms.subject | Diversity | en |
dcterms.subject | PCR | en |
dcterms.subject.materia | Agronomía, reproducción y protección de plantas | es |
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