Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys

cic.isFulltext true es
cic.isPeerReviewed true es
cic.lugarDesarrollo Instituto de Investigación en Informática es
cic.version info:eu-repo/semantics/publishedVersion es
dc.date.accessioned 2015-10-13T14:19:51Z
dc.date.available 2015-10-13T14:19:51Z
dc.identifier.uri https://digital.cic.gba.gob.ar/handle/11746/1215
dc.title Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys en
dc.type Artículo es
dcterms.abstract Paenibacillus larvae, the causal agent of American foulbrood disease in honeybees, acquires tetracycline-resistance via native plasmids carrying known tetracycline-resistance determinants. From three P. larvae tetracycline-resistant strains isolated from honeys, 5-kb-circular plasmids with almost identical sequences, designated pPL373 in strain PL373, pPL374 in strain PL374, and pPL395 in strain PL395, were isolated. These plasmids were highly similar (99%) to small tetracycline- encoding plasmids (pMA67, pBHS24, pBSDMV46A, pDMV2, pSU1, pAST4, and pLS55) that replicate by the rolling circle mechanism. Nucleotide sequences comparisons showed that pPL373, pPL374, and pPL395 mainly differed from the previously reported P. larvae plasmid pMA67 in the oriT region and mob genes. These differences suggest alternative mobilization and/or conjugation capacities. Plasmids pPL373, pPL374, and pPL395 were individually transferred by electroporation and stably maintained in tetracycline-susceptible P. larvae NRRL B-14154, in which they autonomously replicated. The presence of nearly identical plasmids in five different genera of gram-positive bacteria, i.e., Bhargavaea, Bacillus, Lactobacillus, Paenibacillus, and Sporosarcina, inhabiting diverse ecological niches provides further evidence of the genetic transfer of tetracycline resistance among environmental bacteria from soils, food, and marine habitats and from pathogenic bacteria such as P. larvae. en
dcterms.creator.author Alippi, Adriana Mónica es
dcterms.creator.author León, Ignacio E. es
dcterms.creator.author López, Ana C. es
dcterms.extent p. 49-61 es
dcterms.identifier.other DOI: :10.2436/20.1501.01.207 es
dcterms.identifier.other ISSN: 1618-1095 es
dcterms.identifier.url Documento Completo es
dcterms.isPartOf.issue vol. 17, no. 1 es
dcterms.isPartOf.series International Microbiology es
dcterms.issued 2014-03-25
dcterms.language Inglés es
dcterms.license Attribution-NonCommercial-ShareAlike 3.0 Unported (BY-NC-SA 3.0) es
dcterms.publisher Spanish Society for Microbiology (SEM) es
dcterms.subject American foulbrood disease (AFB) es
dcterms.subject Paenibacillus es
dcterms.subject Tetraciclina es
dcterms.subject Abejas es
dcterms.subject Plásmidos es
dcterms.subject.materia Ciencias Agrícolas es
Archivos
Paquete original
Mostrando 1 - 1 de 1
Imagen en miniatura
Nombre:
Alippi-bitstream_3762-A.pdf
Tamaño:
4.53 MB
Formato:
Adobe Portable Document Format
Descripción:
Documento completo