Presence of Fusarium graminearum sensu stricto associated with triticale (× Triticosecale Wittmack) in Argentina

cic.institucionOrigenLaboratorio de Biología Funcional y Biotecnología
cic.isFulltextSI
cic.isPeerReviewedtrue
cic.lugarDesarrolloLaboratorio de Biología Funcional y Biotecnología
cic.parentTypeArtículo
cic.versionPublicada
dc.date.accessioned2023-06-26T17:14:38Z
dc.date.available2023-06-26T17:14:38Z
dc.identifier.urihttps://digital.cic.gba.gob.ar/handle/11746/11963
dc.titlePresence of Fusarium graminearum sensu stricto associated with triticale (× Triticosecale Wittmack) in Argentinaen
dc.typeComunicacion
dcterms.abstractAim of study: To report the occurrence of Fusarium graminearum sensu stricto (s.s.) on triticale grains from field samples in Argentina and the potential mycotoxin production for these isolates. Area of study: Buenos Aires province, Argentina Material and methods: A total of 40 samples from different crops (barley, rye, triticale, and wheat) showing Fusarium head blight symptoms were taken during 2017/2018 harvest season. Colonies with colour and mycelium similar to Fusarium were taken and were morphologically and molecularly identified. The potential to produce deoxynivalenol, 15-acetyl deoxynivalenol and zearalenones was determined. Also, the Koch´s postulates were used to evaluate the pathogenic capacity of the F. graminearum s.s. isolates in triticale. Main results: Two Fusarium isolates were identified morphologically as F. graminearum, which were confirmed molecularly by PCR using the specific Fg16 F/R primers pair and by sequencing red and tri101 genes. The sequences obtained were compared with those available in the NCBI database using BLAST tools, showing 99-100% homology with those belonging to F. graminearum s.s. The results demonstrated that F. graminearum s.s. isolates were pathogenic when triticale spikes were inoculated by spraying under greenhouse conditions. Research highlights: To our knowledge, this is the first time that the presence of F. graminearum s.s. is reported associated with triticale in Argentina.en
dcterms.creator.authorDinolfo, María Inés
dcterms.creator.authorMartínez, Mauro
dcterms.creator.authorCastañares, Eliana
dcterms.creator.authorStenglein, Sebastián
dcterms.identifier.otherDOI: 10.5424/sjar/2020181-14796
dcterms.identifier.urlhttps://revistas.inia.es/index.php/sjar/article/view/14796
dcterms.isPartOf.issuevol. 18, no. 1
dcterms.isPartOf.seriesSpanish Journal of Agricultural Research
dcterms.issued2020
dcterms.languageInglés
dcterms.licenseAttribution 4.0 International (BY 4.0)
dcterms.subjectdeoxynivalenolen
dcterms.subjectmolecular identificationen
dcterms.subjectmycotoxinsen
dcterms.subjectPCRen
dcterms.subjectplant pathogensen
dcterms.subjectsequencingen
dcterms.subjectzearalenoneen
dcterms.subject.materiaCiencias Biológicas

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