DEVDase activity is induced in potato leaves during Phytophthora infestans infection.

cic.isFulltexttruees
cic.isPeerReviewedtruees
cic.lugarDesarrolloUniversidad Nacional de Mar del Plata es
cic.versioninfo:eu-repo/semantics/submittedVersiones
dc.date.accessioned2017-04-05T16:31:17Z
dc.date.available2017-04-05T16:31:17Z
dc.identifier.urihttps://digital.cic.gba.gob.ar/handle/11746/5523
dc.titleDEVDase activity is induced in potato leaves during Phytophthora infestans infection.en
dc.typeArtículoes
dcterms.abstractProgrammed cell death (PCD) occurs in plants, animals and several branches of unicellular eukaryotes as a part of developmental and/or defense processes. Caspase proteases are universal mediators of animal apoptosis, a type of PCD. In plants, there are not animal caspase homologs; therefore, the characterization of caspase-like activities is of considerable importance to our understanding of PCD in plants. Here we report for the first time the involvement of caspase-3-like activity in the resistance mechanism of potato to Phytophthora infestans infection. We showed that disease development in infected potato leaves is dependent of caspase-3-like activity. Unlike plant DEVDases previously reported, this DEVDase activity was sensitive to the serine protease inhibitor PMSF. As reported for other subtilisin- like proteases with caspase activity, potato DEVDase activity was mainly localized in the apoplast. We demonstrated that in total protein extract DEVDase activity accounts for a 60% of serine proten
dcterms.creator.authorFernández, M.B.es
dcterms.creator.authorDaleo, Gustavo Raúles
dcterms.creator.authorGuevara, M.G.es
dcterms.extent7 p.es
dcterms.isPartOf.issuevol. 61es
dcterms.isPartOf.seriesPlant Physiology and Biochemistryes
dcterms.issued2012-10-12
dcterms.languageIngléses
dcterms.licenseAttribution-NonCommercial-NoDerivatives 4.0 International (BY-NC-ND 4.0)es
dcterms.subjectDEVDaseen
dcterms.subjectPlant caspasesen
dcterms.subjectPotatoen
dcterms.subjectP. infestansen
dcterms.subjectApoplastic proteinsen
dcterms.subject.materiaBioquímica y Biología Moleculares
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