Alpha hemolysin induces an increase of erythrocytes calcium: a FLIM 2-photon phasor analysis approach

cic.isFulltexttruees
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cic.lugarDesarrolloUniversidad Nacional de La Plata es
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dc.date.accessioned2016-09-23T15:26:09Z
dc.date.available2016-09-23T15:26:09Z
dc.identifier.urihttps://digital.cic.gba.gob.ar/handle/11746/4298
dc.titleAlpha hemolysin induces an increase of erythrocytes calcium: a FLIM 2-photon phasor analysis approachen
dc.typeArtículoes
dcterms.abstractα-Hemolysin (HlyA) from Escherichia coli is considered as the prototype of a family of toxins called RTX (repeat in toxin), a group of proteins that share genetic and structural features. HlyA is an important virulence factor in E. coli extraintestinal infections, such as meningitis, septicemia and urinary infections. High concentrations of the toxin cause the lysis of several cells such as erythrocytes, granulocytes, monocytes, endothelial and renal epithelial cells of different species. At low concentrations it induces the production of cytokines and apoptosis. Since many of the subcytolytic effects in other cells have been reported to be triggered by the increase of intracellular calcium, we followed the calcium concentration inside the erythrocytes while incubating with sublytic concentrations of HlyA. Calcium concentration was monitored using the calcium indicator Green 1, 2-photon excitation, and fluorescence lifetime imaging microscopy (FLIM). Data were analyzed using the phasor representation. In this report, we present evidence that, at sublytic concentrations, HlyA induces an increase of calcium concentration in rabbit erythrocytes in the first 10 s. Results are discussed in relation to the difficulties of measuring calcium concentrations in erythrocytes where hemoglobin is present, the contribution of the background and the heterogeneity of the response observed in individual cells. © 2011 Sanchez et al.en
dcterms.creator.authorSánchez, Susanaes
dcterms.creator.authorBakás, Laura Susanaes
dcterms.creator.authorGratton, Enricoes
dcterms.creator.authorHerlax, Vanesaes
dcterms.extent9 p.es
dcterms.identifier.otherISSN 1932-6203es
dcterms.identifier.otherDOI: 10.1371/journal.pone.0021127es
dcterms.identifier.urlDocumento completoes
dcterms.isPartOf.issuevol. 6, nº 6es
dcterms.isPartOf.seriesPLoS ONEes
dcterms.issued2011
dcterms.languageIngléses
dcterms.licenseAttribution 4.0 International (BY 4.0)es
dcterms.publisherPlos Onees
dcterms.subjectMicroscopía Fluorescentees
dcterms.subjectCalcioes
dcterms.subjectCitotoxinases
dcterms.subjectMicroscopía Fluorescentees
dcterms.subject.materiaBioquímica y Biología Moleculares
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