Arbuscular mycorrhizal fungi (AMF) are considered as a possible alternative for the biological control of plantparasitic nematodes. The false root-knot nematode Nacobbus aberrans is an endoparasite that induces the formation of galls in plant roots and causes severe damage to crops of economic importance. The purpose of the study was to evaluate the effect of the individual and combined application of two AMF species (Rhizophagus intraradices and Funneliformis mosseae) on the tomato root penetration of N. aberrans second-stage juveniles (J2). Forty-five days after the application of AMF, 300 J2 were inoculated per plant. Tomato roots were analyzed after 4, 8 and 12 days in order to quantify the nematodes that had invaded them. Plants inoculated with AMF presented a lower number of juveniles within the roots compared with non-mycorrhizal plants. No significant differences were observed between the individual and combined application of AMF regarding the number of nematode juveniles. The use of R. intraradices and F. mosseae (both individually and combined) reduced the entry of nematodes in tomato roots. The antagonistic effect of AMF on the invasion of N. aberrans J2 is reported for the first time.