A simple method to assess the oxidative susceptibility of low density lipoproteins

cic.lugarDesarrolloUniversidad Nacional de La Plata es
dc.titleA simple method to assess the oxidative susceptibility of low density lipoproteinsen
dcterms.abstractBackground: Oxidative modification of low density lipoproteins (LDL) is recognized as one of the major processes involved in atherogenesis. The in vitro standardized measurement of LDL oxidative susceptibility could thus be of clinical significance. The aim of the present study was to establish a method which would allow the evaluation of oxidative susceptibility of LDL in the general clinical laboratory. Results: LDL was isolated from human plasma by selective precipitation with amphipathic polymers. The ability of LDL to form peroxides was assessed by measuring thiobarbituric acid reactive substances (TBARS) after incubation with Cu2+ and H2O2. Reaction kinetics showed a three-phase pattern (latency, propagation and decomposition phases) which allowed us to select 150 min as the time point to stop the incubation by cooling and EDTA addition. The mixture Cu 2+/H2O2 yielded more lipoperoxides than each one on its own at the same time end-point. Induced peroxidation was measured in normal subjects and in type 2 diabetic patients. In the control group, results were 21.7 ± 1.5 nmol MDA/mg LDL protein, while in the diabetic group results were significantly increased (39.0 ± 3.0 nmol MDA/mg LDL protein; p < 0.001). Conclusion: a simple and useful method is presented for the routine determination of LDL susceptibility to peroxidation in a clinical laboratory.en
dcterms.creator.authorScoccia, Adriana E.es
dcterms.creator.authorMolinuevo, María Silvinaes
dcterms.creator.authorMcCarthy, Antonio Desmondes
dcterms.creator.authorCortizo, Ana Maríaes
dcterms.extent7 p.es
dcterms.identifier.urlRegistro completoes
dcterms.isPartOf.issuevol. 1es
dcterms.isPartOf.seriesBMC Clinical Pathologyes
dcterms.licenseAttribution 4.0 International (BY 4.0)es
dcterms.subjecthydrogen peroxideen
dcterms.subjectlow density lipoproteinen
dcterms.subjectthiobarbituric acid reactive substanceen
dcterms.subjectchemical reaction kineticsen
dcterms.subjectgenetic susceptibilityen
dcterms.subjectoxidative stressen
dcterms.subject.materiaBioquímica y Biología Moleculares
dcterms.subject.materiaCiencias Naturales y Exactases
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