Reduction of l‑phenylalanine in protein hydrolysates using l‑phenylalanine ammonia‑lyase from Rhodosporidium toruloides
| cic.lugarDesarrollo | Centro de Investigación y Desarrollo en Fermentaciones Industriales | es |
| cic.lugarDesarrollo | Universidad Nacional de La Plata | es |
| cic.version | info:eu-repo/semantics/publishedVersion | es |
| dc.date.accessioned | 2018-05-18T02:20:48Z | |
| dc.date.available | 2018-05-18T02:20:48Z | |
| dc.identifier.uri | https://digital.cic.gba.gob.ar/handle/11746/7750 | |
| dc.title | Reduction of l‑phenylalanine in protein hydrolysates using l‑phenylalanine ammonia‑lyase from Rhodosporidium toruloides | en |
| dc.type | Artículo | es |
| dcterms.abstract | l-Phenylalanine ammonia-lyase (PAL, EC 4.3.1.25) from Rhodosporidium toruloides was utilized to remove l-phenylalanine (l-Phe) from different commercial protein hydrolysates. A casein acid hydrolysate (CAH, l-Phe ~2.28 %) was employed as a model substrate. t-Cinnamic acid resulting from deamination of l-Phe was extracted, analyzed at λ = 290 nm, and used for PAL activity determination. Optimum reaction conditions, optimized using successive Doehlert design, were 35 mg mL-1 of CAH and 800 mU mL-1 of PAL, while temperature and pH were 42 °C and 8.7, respectively. Reaction kinetics of PAL with CAH was determined under optimized conditions. Then, removal of l-Phe from CAH was tested. Results showed that more than 92 % of initial l-Phe was eliminated. Similar results were obtained with other protein hydrolysates. These findings demonstrate that PAL is a useful biocatalyst for l-Phe removal from protein hydrolysates, which can be evaluated as potential ingredients in foodstuffs for PKU patients. | es |
| dcterms.creator.author | Castañeda, María Teresita | es |
| dcterms.creator.author | Adachi, Osao | es |
| dcterms.creator.author | Hours, Roque A. | es |
| dcterms.extent | 9 p. | es |
| dcterms.identifier.other | DOI:10.1007/s10295-015-1664-z | es |
| dcterms.identifier.url | Recurso Completo | es |
| dcterms.isPartOf.issue | vol. 42, no.10 | es |
| dcterms.isPartOf.series | Journal of Industrial Microbiology and Biotechnology | es |
| dcterms.issued | 2015-08-05 | |
| dcterms.language | Inglés | es |
| dcterms.license | Attribution-NonCommercial-NoDerivatives 4.0 International (BY-NC-ND 4.0) | es |
| dcterms.subject | Phenylketonuria | en |
| dcterms.subject | Phenylalanine ammonialyase | en |
| dcterms.subject | Rhodosporidium toruloides | en |
| dcterms.subject | Casein acid hydrolysate | en |
| dcterms.subject | l-Phe removal | en |
| dcterms.subject.materia | Ciencias Químicas | es |
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