Usefulness of Urine as a Sample for Detection of Brucella Spp in Male Canines

cic.institucionOrigenInstituto de Investigación sobre Producción Agropecuaria, Ambiente y Saludes
cic.lugarDesarrolloInstituto de Investigación sobre Producción Agropecuaria, Ambiente y Saludes
dc.titleUsefulness of Urine as a Sample for Detection of Brucella Spp in Male Caninesen
dcterms.abstractUrine was used as a sample and Sensitivity (S), Specificity (Sp) and the positive Likelihood Ratio (LR+) of molecular and serological methods, combined with epidemiology and the clinical symptoms for detection of Brucella spp., were compared in blood and urine samples from 241 male canines. The rapid slide agglutination test together with 2- mercaptoethanol (2-ME RSAT) were used as a screening test, followed by confirmation using an indirect immunoenzymatic assay (iELISA) and bacteriological culture. Results were as follows: Test a) PCR (Polymerase Chain Reaction) of blood compared to blood culture: S 80%, Sp 92%, LR+ 10.32 (CI 5.27-19.20) test b) iELISA compared to blood culture: S 100%, Sp 94%, LR+: 16.57 (CI 9.97-27.53), test c) PCR of urine compared to urine culture: S 100%, Sp 93% (CI 8.36-21.56), LR+: 13.64 (CI 8.36-21.56) test d) iELISA compared to urine culture: S 100%, Sp 93%, LR+: 14.5 (CI 9.03-23.26). We conclude that molecular and serological tests in conjunction with epidemiology are both useful for diagnosis and that both blood and urine samples should be assayed together.en
dcterms.creator.authorBoeri, Eduardo
dcterms.creator.authorLópez, Gustavo Aldoes
dcterms.creator.authorGuido, G.
dcterms.creator.authorElena, Sebastiánes
dcterms.creator.authorWanke, María
dcterms.identifier.otherISSN: 2325-9590es
dcterms.isPartOf.issuevol. 5, no. 4es
dcterms.isPartOf.seriesJournal of Veterinary Science & Medical Diagnosises
dcterms.licenseAttribution-NonCommercial-ShareAlike 4.0 International (BY-NC-SA 4.0)es
dcterms.subjectBrucella canises
dcterms.subjectUrine PCRen
dcterms.subjectBrucella spp urine detectionen
dcterms.subjectMale canine urineen
dcterms.subjectBrucella canis epidemiologyen
dcterms.subjectSerology iELISAen
dcterms.subject.materiaCiencias Veterinariases
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