Breast cancer humoral immune response: involvement of Lewis y through the detection of circulating immune complexes and association with Mucin 1 (MUC1)
| cic.isFulltext | true | es |
| cic.isPeerReviewed | true | es |
| cic.lugarDesarrollo | Universidad Nacional de La Plata | es |
| cic.version | info:eu-repo/semantics/submittedVersion | es |
| dc.date.accessioned | 2016-08-17T14:29:57Z | |
| dc.date.available | 2016-08-17T14:29:57Z | |
| dc.identifier.uri | https://digital.cic.gba.gob.ar/handle/11746/3754 | |
| dc.title | Breast cancer humoral immune response: involvement of Lewis y through the detection of circulating immune complexes and association with Mucin 1 (MUC1) | en |
| dc.type | Artículo | es |
| dcterms.abstract | In cancer patients, MUC1 glycoprotein may carry Lewis y which could be involved in immune response. Purposes: 1- to evaluate the presence of Lewis y and MUC1 in circulating immune complexes (Lewis y/CIC and MUC1/CIC, respectively) and their correlation; 2- to analyze the possible presence of Lewis y in carbohydrate chains of tumoral MUC1 glycoprotein and 3- to correlate serum and tissue parameters considered. Pretreatment serum and tissue breast samples from 76 adenocarcinoma, 34 benign and 36 normal specimens were analyzed. Anti-MUC1 and anti-Lewis y MAbs were employed. To detect Lewis y/CIC and MUC1/CIC, ELISA tests were developed; serum samples containing MUC1 were previously selected by Cancer Associated Serum Antigen (CASA). Immunoprecipitation (IP) was performed in 9 malignant, benign and normal samples and analyzed by SDS-PAGE and Western blot. Lewis y and MUC1 expression was studied by immunohistochemistry (IHC). Statistical analysis was performed employing principal component analysis (PCA), ANOVA, Tukey HSD, Chi square test and classical correlation (p < 0.05). By ELISA, Lewis y/IgM/CIC levels showed statistically significant differences between breast cancer versus benign and normal samples; mean +/- SD values expressed in OD units were: 0.525 +/- 0.304; 0.968 +/- 0.482 and 0.928 +/- 0.447, for breast cancer, benign disease and normal samples, respectively, p < 0.05. Lewis y/IgG/CIC did not show any statistically significant difference. MUC1/IgM/CIC correlated with Lewis y/IgM/CIC. By CASA, 9 samples with MUC1 values above the cut off were selected and IP was performed, followed by SDS-PAGE and Western blot; bands at 200 kDa were obtained with each MAb in all the samples. By IHC, with C14 MAb, 47.5%, 31% and 35% of malignant, benign and normal samples, respectively, showed positive reaction while all the samples were positive with anti-MUC1 MAb; in both cases, with a different pattern of expression between malignant and non malignant samples. Our findings support that in breast cancer there was a limited humoral immune response through Lewis y/IgM/CIC levels detection which correlated with MUC1/IgM/CIC. We also found that Lewis y might be part of circulating MUC1 glycoform structure and also that Lewis y/CIC did not correlate with Lewis y expression. | en |
| dcterms.creator.author | Isla Larrain, Marina Teresita | es |
| dcterms.creator.author | Demichelis, Sandra O. | es |
| dcterms.creator.author | Crespo, Marina | es |
| dcterms.creator.author | Lacunza, Ezequiel | es |
| dcterms.creator.author | Barbera, Alberto | es |
| dcterms.creator.author | Creton, Aldo | es |
| dcterms.creator.author | Terrier, Francisco | es |
| dcterms.creator.author | Segal-Eiras, Amada | es |
| dcterms.creator.author | Croce, María Virginia | es |
| dcterms.extent | 11 p. | es |
| dcterms.identifier.other | 1756-9966 | es |
| dcterms.identifier.url | Registro completo | es |
| dcterms.isPartOf.issue | vol. 28 | es |
| dcterms.isPartOf.series | Journal of Experimental & Clinical Cancer Research | es |
| dcterms.issued | 2009-01-01 | |
| dcterms.language | Inglés | es |
| dcterms.license | Attribution 4.0 International (BY 4.0) | es |
| dcterms.subject | antigen antibody complex | en |
| dcterms.subject | breast tumor | en |
| dcterms.subject | cancer staging | en |
| dcterms.subject | immunology | en |
| dcterms.subject | pathology | en |
| dcterms.subject.materia | Ciencias Médicas y de la Salud | es |
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