Influence of vintage, geographic location and cultivar on the structure of microbial communities associated with the grapevine rhizosphere in vineyards of San Juan Province, Argentina

cic.lugarDesarrolloUniversidad Nacional de Quilmeses
dc.titleInfluence of vintage, geographic location and cultivar on the structure of microbial communities associated with the grapevine rhizosphere in vineyards of San Juan Province, Argentinaen
dcterms.abstractSoil microbiomes, as a primary reservoir for plant colonizing fungi and bacteria, play a major role in determining plant productivity and preventing invasion by pathogenic microorganisms. The use of 16S rRNA and ITS high-throughput amplicon sequencing for analysis of complex microbial communities have increased dramatically in recent years, establishing links between wine specificity and, environmental and viticultural factors, which are framed into the elusive terroir concept. Given the diverse and complex role these factors play on microbial soil structuring of agricultural crops, the main aim of this study is to evaluate how external factors, such as vintage, vineyard location, cultivar and soil characteristics, may affect the diversity of the microbial communities present. Additionally, we aim to compare the influence these factors have on the structuring of bacterial and fungal populations associated with Malbec grapevine rhizosphere with that of the more widespread Cabernet Sauvignon grapevine cultivar. Samples were taken from Malbec and Cabernet Sauvignon cultivars from two different vineyards in the San Juan Province of Argentina. Total DNA extracts from the rhizosphere soil samples were sequenced using Illumina’s Miseq technology, targeting the V3-V4 hypervariable 16S rRNA region in prokaryotes and the ITS1 region in yeasts. The major bacterial taxa identified were Proteobacteria, Bacteroidetes and Firmicutes, while the major fungal taxa were Ascomycetes, Basidiomycetes, Mortierellomycetes and a low percentage of Glomeromycetes. Significant differences in microbial community composition were found between vintages and vineyard locations, whose soils showed variances in pH, organic matter, and content of carbon, nitrogen, and absorbable phosphorus.en
dcterms.creator.authorOyuela Aguilar, Mónicaes
dcterms.creator.authorGobbi, Alexes
dcterms.creator.authorBrowne, Patrick
dcterms.creator.authorEllegaard-Jensen, Leaes
dcterms.creator.authorHansen, Lars Hestbjerges
dcterms.creator.authorSemorile, Liliana Carmenes
dcterms.creator.authorPistorio, Marianoes
dcterms.isPartOf.issuevol. 15, no. 2es
dcterms.isPartOf.seriesPlos Onees
dcterms.licenseAttribution 4.0 International (BY 4.0)es
dcterms.subjectInfluence of vintageen
dcterms.subjectMicrobial communitiesen
dcterms.subjectVineyard locationen
dcterms.subjectCultivar and soil characteristicsen
dcterms.subject.materiaBioquímica y Biología Moleculares
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