Artículos y presentaciones en Congresos

URI https://digital.cic.gba.gob.ar/handle/11746/4464

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  • Artículo
    Acceso Abierto
    Microbial diversity of the soil, rhizosphere and wine from an emerging wine-producing region of Argentina
    (2022) Rivas, Gabriel A.; Semorile, Liliana Carmen; Delfederico, Lucrecia
    The aim of this study was to characterize the microbial communities of Malbec vineyards recently established in a re-emerging wine region of Argentina. We studied the wine microbiota at different fermentation stages and the soil and rhizosphere microbial communities of two vineyards. A next-generation sequencing (NGS) approach was used to identify bacterial and fungal communities. The soil and rhizosphere samples showed a predominance of the phyla Proteobacteria and Actinobacteria. The order Rhizobiales stood out in the soil and rhizosphere of the two vineyards analyzed. Members of this order are recognized for their plant-growth promotion properties. Regarding fungal communities, Ascomycota and Basidiomycota were the most abundant phyla. The high abundance of the genus Ilyonectria in one of the vineyards may have impact on the health of vines. In wine samples, we detected low levels of lactic acid bacteria and the persistence of acetic acid bacteria (AAB) throughout the fermentation process, although there were no discernible effects on the acidity of wine. The results achieved could allow winemakers to improve the vineyard management practices and the fermentation process to favor the growth of microorganisms potentially beneficial for the health of the vines and the wine quality, while maintaining the regional microbial biodiversity.
  • Artículo
    Acceso Abierto
    Influence of climate on soil and wine bacterial diversity on a vineyard in a non-traditional wine region in Argentina wine region
    (2021) Rivas, Gabriel A.; Guillade, Andrea C.; Semorile, Liliana Carmen; Delfederico, Lucrecia
    Argentina is the fifth world-wide wine producer, with an area of emerging importance in the Southwest of Buenos Aires Province, where climatic conditions are rather challenging. We studied the variations in soil and wine bacterial diversity through three consecutive vintages, and how climatic conditions affected said diversity. During the years of our study there were two harsh climatic events, a prolonged drought that extended over two vegetative periods, and an unseasonable spring frost in 2017. We found that the bacterial diversity reacted to these climatic events, given that there was a shift in the taxa exclusive to soil and wine, and shared by both, through time. Our results show a core of microorganisms in soil as well as in wine, belonging to different phyla that are conserved across the vintage years. A trend to an enrichment in Actinobacteria was detected in soil samples, whereas a high relative abundance of the Acetobacteraceae family and a scarcity of Lactic Acid Bacteria (LAB) were detected in the wine samples. We believe our results contribute to a better understanding of the impact of climatic conditions on the soil and wine microbiota, and can provide vintners with valuable knowledge for improving their wine production.
  • Artículo
    Acceso Abierto
    Influence of vintage, geographic location and cultivar on the structure of microbial communities associated with the grapevine rhizosphere in vineyards of San Juan Province, Argentina
    (2020) Oyuela Aguilar, Mónica; Gobbi, Alex; Browne, Patrick D.; Ellegaard-Jensen, Lea; Hansen, Lars Hestbjerg; Semorile, Liliana Carmen; Pistorio, Mariano
    Soil microbiomes, as a primary reservoir for plant colonizing fungi and bacteria, play a major role in determining plant productivity and preventing invasion by pathogenic microorganisms. The use of 16S rRNA and ITS high-throughput amplicon sequencing for analysis of complex microbial communities have increased dramatically in recent years, establishing links between wine specificity and, environmental and viticultural factors, which are framed into the elusive terroir concept. Given the diverse and complex role these factors play on microbial soil structuring of agricultural crops, the main aim of this study is to evaluate how external factors, such as vintage, vineyard location, cultivar and soil characteristics, may affect the diversity of the microbial communities present. Additionally, we aim to compare the influence these factors have on the structuring of bacterial and fungal populations associated with Malbec grapevine rhizosphere with that of the more widespread Cabernet Sauvignon grapevine cultivar. Samples were taken from Malbec and Cabernet Sauvignon cultivars from two different vineyards in the San Juan Province of Argentina. Total DNA extracts from the rhizosphere soil samples were sequenced using Illumina’s Miseq technology, targeting the V3-V4 hypervariable 16S rRNA region in prokaryotes and the ITS1 region in yeasts. The major bacterial taxa identified were Proteobacteria, Bacteroidetes and Firmicutes, while the major fungal taxa were Ascomycetes, Basidiomycetes, Mortierellomycetes and a low percentage of Glomeromycetes. Significant differences in microbial community composition were found between vintages and vineyard locations, whose soils showed variances in pH, organic matter, and content of carbon, nitrogen, and absorbable phosphorus.
  • Artículo
    Acceso Abierto
    Aplicación de un Enfoque Metagenómico al Estudio de la Diversidad Bacteriana asociada a Suelo, Rizósfera y Vinos de la Región Bonaerense Argentina
    (2019) Rivas, Gabriel Alejandro; Delfederico, Lucrecia; Semorile, Liliana Carmen
    Durante la fermentación malolática (FML) las bacterias del ácido láctico (BAL) decarboxilan el ácido L-málico transformándolo en ácido L-láctico y contribuyen a la calidad del vino al reducir su acidez y aportar a su aroma y sabor. De este modo, las BAL aportan al terroir característico de cada vino, y su diversidad en el viñedo dependerá de factores como clima, suelo, varietal vínico y prácticas culturales y de vinificación. En la región vitícola bonaerense (Argentina) no existen aún estudios sobre la diversidad microbiológica asociada a suelos, vides, mostos y vinos, conocimiento crucial para seleccionar cepas indígenas con fines enológicos. Para iniciar este análisis se realizó la secuenciación masiva del gen 16S rRNA de muestras de suelo, rizósfera, mosto y vinos de dos bodegas del SO bonaerense (Saldungaray y Al Este, Buenos Aires, Argentina), obteniéndose 123.379 secuencias y 5.323 OTUs. Se observaron índices de diversidad elevados, con diferencias significativas entre muestras de suelo y rizósfera respecto de mosto y vino. Los análisis taxonómicos mostraron como filo mayoritario en suelo y rizósfera a Proteobacteria (especialmente Alphaproteobacteria, con predominancia de Rhizobiales), y en mosto y vino a Proteobacteria, con predominancia de las Alphaproteobacteria Rhodospirillales y Sphingomonadales. Estos hallazgos, constituyen un primer aporte al conocimiento de las comunidades bacterianas de una región vitivinícola en desarrollo y contribuirán a optimizar procesos productivos y prácticas agrícolas sustentables.
  • Artículo
    Acceso Abierto
    Inocentes y arriesgadas: estudio de la cobertura mediática del doble femicidio de Coni y Menegazzo
    (2017) Fernández López, Ludmila
    Este artículo se propone analizar la cobertura mediática del doble femicidio de María José Coni y Marina Menegazzo, ocurrido en febrero de 2016 en Ecuador. Se releva un conjunto de notas correspondientes a tres medios masivos, donde se indaga por los modos de nombrar a las víctimas y el énfasis puesto en determinados aspectos como el lugar de los hechos, la juventud y el ambiente festivo, entre otros. Desde la teoría de los pánicos morales, articulada con una lectura feminista del material, se sostiene que estos relatos no sólo invisibilizaron la trama de violencia machista que subyace a cualquier femicidio, sino también alentaron los miedos sobre las formas de habitar los momentos de ocio entre las y los jóvenes, ubicando como único destinatario posible del discurso periodístico al público adulto.