Peptides from Amaranth controlled the NF-κB pathway activation on epithelial cells and suppressed intestinal inflammation
cic.isFulltext | true | es |
cic.isPeerReviewed | true | es |
cic.lugarDesarrollo | Universidad Nacional de La Plata | es |
cic.version | info:eu-repo/semantics/submittedVersion | es |
dc.date.accessioned | 2019-01-02T12:48:36Z | |
dc.date.available | 2019-01-02T12:48:36Z | |
dc.identifier.uri | https://digital.cic.gba.gob.ar/handle/11746/8700 | |
dc.title | Peptides from Amaranth controlled the NF-κB pathway activation on epithelial cells and suppressed intestinal inflammation | en |
dc.type | Documento de conferencia | es |
dcterms.abstract | Biological, nutritional and health benefits of amaranth have been highlighted in the last years. Proteins of amaranth exert anti-hypertensive, anti-oxidant, anti-thrombotic and anti-proliferative effects. The aim of this study was to analyze the anti-inflammatory effect of peptides from amaranth on NF-kB-intracellular pathway activation in intestinal epithelial cells, and in experimental intestinal inflammation, such as colitis and food allergy. Colon cell lines (Caco-2 and Caco-luc) were cultured with flagellin and amaranth peptides. CCL20-expression was evaluated by qPCR and NF-κB modulation was evaluated by light emission and qPCR, along with p65-nuclear traslocation. In vivo studies included the oral administration of a formulation containing the peptide during the allergic sensitization or the colitis induction phase in Balb/c mice. Treatment efficacy was in vivo and in vitro evaluated. We found several peptides with anti-inflammatory capacity and we selected that with the highest ability to suppress cell activation (decrease in CCL20 and light emission p<0.05). In vivo studies showed, an amelioration of the clinical score (p<0.01) in the food allergy mouse model, with inhibition of specific-IgE secretion (p<0.05) and negativitization of the cutaneous test (mean increase in footpad thickness control: 0.6mm vs peptide-treated: 0.3mm; p<0.05); intestinal nf-kb gene expression was reduced (fold change=3; p<0.01) along with up-regulation of tfg-b and foxp3. In the colitis mouse model, we found a decrease of the histologic score with a decrease expression and production of pro-inflammatory cytokines (IL-1b, TNF and IFNg, p<0.05) and a decrease in the myeloperoxidase activity in the peptide-treated group compared to control (p<0.05). NF-κB pathway was also abrogated in the gut. In conclusion, our findings indicated that peptides from amaranth endowed mucosal anti-inflammatory properties that suppressed the intestinal activation of NF-kB in Th1- and Th2-mediated inflammation. These findings led us to propose that this peptide might be included in the composition of a functional food | en |
dcterms.creator.author | Smaldini, Paola | es |
dcterms.creator.author | Quereda, Micaela | es |
dcterms.creator.author | Rizzo, Gastón | es |
dcterms.creator.author | Orsini Delgado, Maria Lucia | es |
dcterms.creator.author | Moronta, Julian | es |
dcterms.creator.author | Añón, María Cristina | es |
dcterms.creator.author | Docena, Guillermo | es |
dcterms.extent | 1 p. | es |
dcterms.isPartOf.issue | Reunión Conjunta de la Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Fisiología (SAFIS) (Mar del Plata, 2018) | es |
dcterms.isPartOf.series | Reunión Conjunta de la Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Fisiología (SAFIS) | es |
dcterms.issued | 2018-11-15 | |
dcterms.language | Inglés | es |
dcterms.license | Attribution-NonCommercial-ShareAlike 4.0 International (BY-NC-SA 4.0) | es |
dcterms.subject | Mucosal Immunology | en |
dcterms.subject | Peptides from Amaranth | en |
dcterms.subject | intestinal inflammation | en |
dcterms.subject.materia | Ciencias de la Salud | es |
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