Peptides from Amaranth controlled the NF-κB pathway activation on epithelial cells and suppressed intestinal inflammation

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cic.lugarDesarrolloUniversidad Nacional de La Plataes
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dc.date.accessioned2019-01-02T12:48:36Z
dc.date.available2019-01-02T12:48:36Z
dc.identifier.urihttps://digital.cic.gba.gob.ar/handle/11746/8700
dc.titlePeptides from Amaranth controlled the NF-κB pathway activation on epithelial cells and suppressed intestinal inflammationen
dc.typeDocumento de conferenciaes
dcterms.abstractBiological, nutritional and health benefits of amaranth have been highlighted in the last years. Proteins of amaranth exert anti-hypertensive, anti-oxidant, anti-thrombotic and anti-proliferative effects. The aim of this study was to analyze the anti-inflammatory effect of peptides from amaranth on NF-kB-intracellular pathway activation in intestinal epithelial cells, and in experimental intestinal inflammation, such as colitis and food allergy. Colon cell lines (Caco-2 and Caco-luc) were cultured with flagellin and amaranth peptides. CCL20-expression was evaluated by qPCR and NF-κB modulation was evaluated by light emission and qPCR, along with p65-nuclear traslocation. In vivo studies included the oral administration of a formulation containing the peptide during the allergic sensitization or the colitis induction phase in Balb/c mice. Treatment efficacy was in vivo and in vitro evaluated. We found several peptides with anti-inflammatory capacity and we selected that with the highest ability to suppress cell activation (decrease in CCL20 and light emission p<0.05). In vivo studies showed, an amelioration of the clinical score (p<0.01) in the food allergy mouse model, with inhibition of specific-IgE secretion (p<0.05) and negativitization of the cutaneous test (mean increase in footpad thickness control: 0.6mm vs peptide-treated: 0.3mm; p<0.05); intestinal nf-kb gene expression was reduced (fold change=3; p<0.01) along with up-regulation of tfg-b and foxp3. In the colitis mouse model, we found a decrease of the histologic score with a decrease expression and production of pro-inflammatory cytokines (IL-1b, TNF and IFNg, p<0.05) and a decrease in the myeloperoxidase activity in the peptide-treated group compared to control (p<0.05). NF-κB pathway was also abrogated in the gut. In conclusion, our findings indicated that peptides from amaranth endowed mucosal anti-inflammatory properties that suppressed the intestinal activation of NF-kB in Th1- and Th2-mediated inflammation. These findings led us to propose that this peptide might be included in the composition of a functional fooden
dcterms.creator.authorSmaldini, Paolaes
dcterms.creator.authorQuereda, Micaelaes
dcterms.creator.authorRizzo, Gastónes
dcterms.creator.authorOrsini Delgado, Maria Luciaes
dcterms.creator.authorMoronta, Julianes
dcterms.creator.authorAñón, María Cristinaes
dcterms.creator.authorDocena, Guillermoes
dcterms.extent1 p.es
dcterms.isPartOf.issueReunión Conjunta de la Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Fisiología (SAFIS) (Mar del Plata, 2018)es
dcterms.isPartOf.seriesReunión Conjunta de la Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Fisiología (SAFIS)es
dcterms.issued2018-11-15
dcterms.languageIngléses
dcterms.licenseAttribution-NonCommercial-ShareAlike 4.0 International (BY-NC-SA 4.0)es
dcterms.subjectMucosal Immunologyen
dcterms.subjectPeptides from Amaranthen
dcterms.subjectintestinal inflammationen
dcterms.subject.materiaCiencias de la Saludes
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