Insulin-deficient diabetes-induced bone microarchitecture alterations are associated with a decrease in the osteogenic potential of bone marrow progenitor cells: preventive effects of metformin
| cic.institucionOrigen | Laboratorio de Investigación en Osteopatías y Metabolismo Mineral | es |
| cic.isFulltext | true | es |
| cic.isPeerReviewed | true | es |
| cic.lugarDesarrollo | Laboratorio de Investigación en Osteopatías y Metabolismo Mineral | es |
| cic.version | info:eu-repo/semantics/publishedVersion | es |
| dc.date.accessioned | 2021-10-07T17:40:58Z | |
| dc.date.available | 2021-10-07T17:40:58Z | |
| dc.identifier.uri | https://digital.cic.gba.gob.ar/handle/11746/11321 | |
| dc.title | Insulin-deficient diabetes-induced bone microarchitecture alterations are associated with a decrease in the osteogenic potential of bone marrow progenitor cells: preventive effects of metformin | en |
| dc.type | Artículo | es |
| dcterms.abstract | Aims: Diabetes mellitus is associated with metabolic bone disease and increased lowimpact fractures. The insulin-sensitizer metformin possesses in vitro, in vivo and ex vivo osteogenic effects, although this has not been adequately studied in the context of diabetes. We evaluated the effect of insulin-deficient diabetes and/or metformin on bone microarchitecture, on osteogenic potential of bone marrow progenitor cells (BMPC) and possible mechanisms involved. Methods: Partially insulin-deficient diabetes was induced in rats by nicotinamide/streptozotocin- injection, with or without oral metformin treatment. Femoral metaphysis microarchitecture, ex vivo osteogenic potential of BMPC, and BMPC expression of Runx-2, PPARg and receptor for advanced glycation endproducts (RAGE) were investigated. Results: Histomorphometric analysis of diabetic femoral metaphysis demonstrated a slight decrease in trabecular area and a significant reduction in osteocyte density, growth plate height and TRAP (tartrate-resistant acid phosphatase) activity in the primary spongiosa. BMPC obtained from diabetic animals showed a reduction in Runx-2/PPARg ratio and in their osteogenic potential, and an increase in RAGE expression. Metformin treatment prevented the diabetes-induced alterations in bone micro-architecture and BMPC osteogenic potential. Conclusion: Partially insulin-deficient diabetes induces deleterious effects on long-bone micro-architecture that are associated with a decrease in BMPC osteogenic potential, which could be mediated by a decrease in their Runx-2/PPARg ratio and up-regulation of RAGE. These diabetes-induced alterations can be totally or partially prevented by oral administration of metformin. | en |
| dcterms.creator.author | Tolosa, María José | es |
| dcterms.creator.author | Chuguransky, Sara Rocío | es |
| dcterms.creator.author | Sedlinsky, Claudia | es |
| dcterms.creator.author | Schurman, León | es |
| dcterms.creator.author | McCarthy, Antonio Desmond | es |
| dcterms.creator.author | Molinuevo, María Silvina | es |
| dcterms.creator.author | Cortizo, Ana María | es |
| dcterms.extent | 177-186 | es |
| dcterms.identifier.other | doi:10.1016/j.diabres.2013.05.016 | es |
| dcterms.identifier.other | ISSN:0168-8227 | es |
| dcterms.isPartOf.issue | vol. 101, no. 2 | es |
| dcterms.isPartOf.series | Diabetes Research and Clinical Practice | es |
| dcterms.issued | 2013 | |
| dcterms.language | Inglés | es |
| dcterms.license | Attribution-NonCommercial-ShareAlike 4.0 International (BY-NC-SA 4.0) | es |
| dcterms.subject | Diabetes Mellitus | en |
| dcterms.subject | Metformin | en |
| dcterms.subject | Bone microarchitecture | en |
| dcterms.subject | Bone marrow progenitor cells | en |
| dcterms.subject.materia | Ciencias Médicas | es |
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