Fumarate/ceramic composite based Scaffolds for tissue engineering: evaluation of hydrophylicity, degradability, toxicity and biocompatibility

cic.isFulltexttruees
cic.isPeerReviewedtruees
cic.lugarDesarrolloUniversidad Nacional de La Plata es
cic.versioninfo:eu-repo/semantics/submittedVersiones
dc.date.accessioned2016-11-18T17:23:23Z
dc.date.available2016-11-18T17:23:23Z
dc.identifier.urihttps://digital.cic.gba.gob.ar/handle/11746/4756
dc.titleFumarate/ceramic composite based Scaffolds for tissue engineering: evaluation of hydrophylicity, degradability, toxicity and biocompatibilityen
dc.typeArtículoes
dcterms.abstractThe present study was designed to investigate the possible cytotoxicity and biocompatibility of scaffolds based on previously characterized polymeric materials including poly- -caprolactone (PCL) or polydiisopropyl fumarate (blended or on their own), with or without hydroxyapatite (HAP). Water contact angle was also evaluated to determine the hydrophylicity of each scaffold. Degradation of different scaffolds was evaluated after a 10-week incubation in Dulbecco’s modified eagle medium (DMEM) supplemented with 5% (v/v) fetal bovine serum (FBS). Bone Marrow Stromal Cells (MSC) were grown on different scaffolds in an osteogenic medium, after which alkaline phosphatase activity (ALP) was evaluated. ALP activity increased when MSC were grown on PCL+HAP or Blend+HAP, as compared to PCL or Blend without HAP. The effect of different scaffolds on the proliferation of the macrophage cell line RAW 264.7, production of nitric oxide (NO) and secretion of pro-inflammatory cytokines was examined. After 72 h, macrophages proliferated equally well on all scaffolds, maintaining a rounded morphology. None of the investigated scaffolds induced production of NO or cytokine release into the culture media, suggesting an absence of cytotoxicity. Therefore, these polymer- and HAP-based scaffolds could potentially be used as bone substitute materials.en
dcterms.creator.authorFernández, Juan Manueles
dcterms.creator.authorCortizo, María Susanaes
dcterms.creator.authorCortizo, Ana Maríaes
dcterms.extent8 p.es
dcterms.isPartOf.issuevol. 4, no. 1-8es
dcterms.isPartOf.seriesJournal of Biomaterials and Tissue Engineeringes
dcterms.issued2014
dcterms.languageIngléses
dcterms.licenseAttribution 4.0 International (BY 4.0)es
dcterms.subjectcytotoxicityen
dcterms.subjectbone tissue engineeringen
dcterms.subjectbone marrow stromal cellsen
dcterms.subjectRAW 264.7 macrophagesen
dcterms.subjectpoly-epsilon-caprolactoneen
dcterms.subjectpolydiisopropyl fumarateen
dcterms.subject.materiaCiencias Químicases
Archivos
Paquete original
Mostrando1 - 1 de 1
Imagen en miniatura
Nombre:
088 - Fernandez JM-J Biomater Tissue Eng2014 ARTICULO.pdf-PDFA.pdf
Tamaño:
828.99 KB
Formato:
Adobe Portable Document Format
Descripción:
Documento completo