A conventional PCR technique to detect Septoria tritici in wheat seeds
| cic.isFulltext | true | es |
| cic.isPeerReviewed | true | es |
| cic.lugarDesarrollo | Centro de Investigaciones de Fitopatología | es |
| cic.version | info:eu-repo/semantics/submittedVersion | es |
| dc.date.accessioned | 2018-03-15T16:36:16Z | |
| dc.date.available | 2018-03-15T16:36:16Z | |
| dc.identifier.uri | https://digital.cic.gba.gob.ar/handle/11746/6993 | |
| dc.title | A conventional PCR technique to detect Septoria tritici in wheat seeds | en |
| dc.type | Artículo | es |
| dcterms.abstract | A PCR assay was developed for detection of wheat seed naturally contaminated with <em>Septoria tritici. S. tritici </em>specific primers were derived from strict alignment of ITS and a-tubulin sequences of the pathogen. The specificity of four sets of synthesised oligonucleotide pairs (A, B, C and D) were tested using isolates from <em>S. tritici</em>, other selected fungi and wheat seeds. A single DNA fragment was amplified from <em>S. tritici i</em>solates with all primer pairs, whereas no product was generated from otherDNAsources.<em> S. tritici </em>was also detected in wheat seed lots collected from plants with variable pycnidial coverage on the upper two leaves. PCR detection of as little as 0.5 pg of <em>S. tritici</em> genomic DNAwas possible. This is the first report on the detection of<em> S. tritici </em>DNA in naturally infested wheat seeds. This PCR based assay is simple, rapid, specific, sensitive and suitable for routine detection of the wheat pathogen in infested wheat seeds. | en |
| dcterms.creator.author | Consolo, Verónica Fabiana | es |
| dcterms.creator.author | Albani, C.M. | es |
| dcterms.creator.author | Berón, C.M. | es |
| dcterms.creator.author | Salerno, Graciela Lidia | es |
| dcterms.creator.author | Cordo, Cristina Alicia | es |
| dcterms.extent | 6 p. | es |
| dcterms.identifier.other | https://doi.org/10.1071/AP08099 | es |
| dcterms.identifier.url | Recurso Completo | es |
| dcterms.isPartOf.issue | vol. 38, no. 3 | es |
| dcterms.isPartOf.series | Australasian Plant Pathology | es |
| dcterms.issued | 2009-05 | |
| dcterms.language | Inglés | es |
| dcterms.license | Attribution-NonCommercial-NoDerivatives 4.0 International (BY-NC-ND 4.0) | es |
| dcterms.subject | Mycosphaerella graminicola | en |
| dcterms.subject | seedborne pathogen | en |
| dcterms.subject | Septoria leaf blotch | en |
| dcterms.subject | wheat | en |
| dcterms.subject.materia | Agronomía, reproducción y protección de plantas | es |
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