Complete genome amplification of Equine influenza virus subtype 2
| cic.isFulltext | true | es |
| cic.isPeerReviewed | true | es |
| cic.lugarDesarrollo | Universidad Nacional de La Plata | es |
| cic.parentType | Articulo | |
| cic.version | info:eu-repo/semantics/publishedVersion | es |
| dc.date.accessioned | 2016-10-06T14:57:46Z | |
| dc.date.available | 2016-10-06T14:57:46Z | |
| dc.identifier.uri | https://digital.cic.gba.gob.ar/handle/11746/4379 | |
| dc.title | Complete genome amplification of Equine influenza virus subtype 2 | en |
| dc.type | Artículo | es |
| dcterms.abstract | This work reports a method for rapid amplification of the complete genome of equine influenza virus subtype 2 (H3N8). A ThermoScriptTM reverse transcriptase instead of the avian myeloblastosis virus reverse transcriptase or Moloney murine leukemia virus reverse transcriptase was used. This enzyme has demonstrated higher thermal stability and is described as suitable to make long cDNA with a complex secondary structure. The product obtained by this method can be cloned, used in later sequencing reactions or nested-PCR with the purpose of achieving a rapid diagnosis and characterization of the equine influenza virus type A. This detection assay might be a valuable tool for diagnosis and screening of field samples as well as for conducting molecular studies. | en |
| dcterms.alternative | Amplificación del genoma completo del subtipo 2 del virus de la influenza equina | es |
| dcterms.creator.author | Sguazza, G. H. | es |
| dcterms.creator.author | Fuentealba, N. A. | es |
| dcterms.creator.author | Tizzano, Marco Antonio | es |
| dcterms.creator.author | Galosi, Cecilia Mónica | es |
| dcterms.creator.author | Pecoraro, M. R. | es |
| dcterms.extent | p. 207-211 | es |
| dcterms.identifier.other | ISSN 0325-7250417 | es |
| dcterms.identifier.url | Documento completo | es |
| dcterms.isPartOf.issue | vol. 41, nº 4 | es |
| dcterms.isPartOf.series | Revista Argentina de Microbiologia | es |
| dcterms.issued | 2009 | |
| dcterms.language | Inglés | es |
| dcterms.license | Attribution-NonCommercial 4.0 International (BY-NC 4.0) | es |
| dcterms.publisher | Asociación Argentina de Microbiología | es |
| dcterms.subject | virus de la influenza equina | es |
| dcterms.subject | Genoma | es |
| dcterms.subject | Diagnóstico | es |
| dcterms.subject | RT-PCR | es |
| dcterms.subject.materia | Ciencias Veterinarias | es |
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